Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Department of Pharmacy, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China.
Oncol Rep. 2019 Nov;42(5):1915-1923. doi: 10.3892/or.2019.7292. Epub 2019 Aug 23.
As a member of the myotubularin family, myotubularin related protein 3 (MTMR3) has been demonstrated to participate in tumor development, including oral and colon cancer. However, little is known about its functional roles in breast cancer. In the present study, the expression of MTMR3 in breast cancer was evaluated by immunohistochemical staining of tumor tissues from 172 patients. Online data was then used for survival analysis from the PROGgeneV2 database. In vitro, MTMR3 expression was silenced in MDA‑MB‑231 cells via lentiviral shRNA transduction. MTT, colony formation and flow cytometry assays were performed in the control and MTMR3‑silenced cells to evaluate the cell growth, proliferation and cell cycle phase distribution, respectively. Western blotting was used to evaluate the protein expression levels of autophagy‑related markers. The results demonstrated that the expression of MTMR3 in breast cancer tissues was significantly increased compared with adjacent normal tissues. MTMR3 was highly expressed in triple‑negative breast cancer and was associated with disease recurrence. MTMR3 knockdown in MDA‑MB‑231 cells inhibited cell proliferation and induced cell cycle arrest and autophagy. The present results indicated that MTMR3 may have an important role in promoting the progression of breast cancer, and its inhibition may serve as a promising therapeutic target for breast cancer treatment.
作为肌微管相关蛋白家族的一员，肌微管相关蛋白 3（MTMR3）已被证实参与肿瘤的发展，包括口腔癌和结肠癌。然而，其在乳腺癌中的功能作用知之甚少。在本研究中，通过对 172 例患者的肿瘤组织进行免疫组织化学染色，评估了 MTMR3 在乳腺癌中的表达。随后，利用 PROGgeneV2 数据库中的在线数据进行生存分析。在体外，通过慢病毒 shRNA 转导沉默 MDA-MB-231 细胞中的 MTMR3 表达。在对照和 MTMR3 沉默的细胞中，通过 MTT、集落形成和流式细胞术检测分别评估细胞生长、增殖和细胞周期分布。Western blot 用于评估自噬相关标志物的蛋白表达水平。结果表明，与相邻正常组织相比，乳腺癌组织中 MTMR3 的表达显著增加。MTMR3 在三阴性乳腺癌中高表达，与疾病复发相关。在 MDA-MB-231 细胞中敲低 MTMR3 抑制细胞增殖并诱导细胞周期停滞和自噬。本研究结果表明，MTMR3 可能在促进乳腺癌进展中发挥重要作用，抑制其表达可能成为治疗乳腺癌的有前途的治疗靶点。
