Department of Breast Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an, Shaanxi 710061, P.R. China.
Oncol Rep. 2019 Nov;42(5):1699-1708. doi: 10.3892/or.2019.7297. Epub 2019 Aug 29.
Accumulated evidence indicates that aberrant microRNAs (miRNAs) expression plays an important role in the initiation and progression of various cancers, including breast cancer. Previous studies suggested that miR‑139‑3p might serve as a tumor suppressor and is downregulated in several cancer types. However, the expression patterns and exact role of miR‑139‑3p in breast cancer remain to be elucidated. In this study, we aimed to analyze the effect of miR‑139‑3p on the progression of breast cancer and the mechanism involved. Through bioinformatics analysis and in vitro experimental studies, we found that miR‑139‑3p was decreased in breast cancer tissues and cell lines, and decreased miR‑139‑3p is associated with a poor prognosis in breast cancer patients. Overexpression of miR‑139‑3p by transfection significantly inhibits cell proliferation, migration, and invasion of breast cancer cells. Bioinformatics analysis and luciferase reporter gene assay confirmed that RAB1A was a potential target of miR‑139‑3p. Furthermore, overexpression of RAB1A counteracted the suppressing effects of miR‑139‑3p on breast cancer cell migration, invasion and epithelial‑to‑mesenchymal transition (EMT). Taken together, these data suggest that miR‑139‑3p plays a tumor suppressive role in breast cancer by targeting RAB1A and may serve as a potential new biomarker for breast cancer prognosis.
已有大量证据表明，异常表达的 microRNAs（miRNAs）在包括乳腺癌在内的多种癌症的发生和发展中起着重要作用。先前的研究表明，miR-139-3p 可能作为一种肿瘤抑制因子，在几种癌症类型中下调。然而，miR-139-3p 在乳腺癌中的表达模式和确切作用仍有待阐明。在本研究中，我们旨在分析 miR-139-3p 对乳腺癌进展的影响及其涉及的机制。通过生物信息学分析和体外实验研究，我们发现 miR-139-3p 在乳腺癌组织和细胞系中表达下调，且 miR-139-3p 下调与乳腺癌患者预后不良相关。通过转染过表达 miR-139-3p 可显著抑制乳腺癌细胞的增殖、迁移和侵袭。生物信息学分析和荧光素酶报告基因检测证实 RAB1A 是 miR-139-3p 的一个潜在靶基因。此外，过表达 RAB1A 可逆转 miR-139-3p 对乳腺癌细胞迁移、侵袭和上皮间质转化（EMT）的抑制作用。综上所述，这些数据表明，miR-139-3p 通过靶向 RAB1A 在乳腺癌中发挥肿瘤抑制作用，可能成为乳腺癌预后的潜在新生物标志物。
