LFB Biotech, Les Ulis, France.
JAM Conseil, Neuilly-sur-Seine, France.
Adv Biochem Eng Biotechnol. 2020;174:93-139. doi: 10.1007/10_2019_106.
Aptamers are oligonucleotide molecules able to recognize very specifically proteins. Among the possible applications, aptamers have been used for affinity chromatography with effective results and advantages over most advanced protein separation technologies. This chapter first discusses the context of the affinity chromatography with aptamer ligands. With the adaptation of SELEX, the chemical modifications of aptamers to comply with the covalent coupling and the separation process are then extensively presented. A focus is then made about the most important applications for protein separation with real-life examples and the comparison with immunoaffinity chromatography. In spite of well-advanced demonstrations and the extraordinary potential developments, a significant optimization work is still due to deserve large-scale applications with all necessary validations. Graphical Abstract Aptamer-protein complexes by X-ray crystallography.
适体是能够非常特异性识别蛋白质的寡核苷酸分子。在可能的应用中，适体已被用于亲和层析，取得了非常有效的结果，并具有优于大多数先进的蛋白质分离技术的优势。本章首先讨论了带有适体配体的亲和层析的背景。通过对 SELEX 的适应，然后广泛介绍了适体的化学修饰以符合共价偶联和分离过程。然后重点介绍了蛋白质分离的最重要应用，包括实际例子和与免疫亲和层析的比较。尽管已经有了先进的演示和非凡的潜在发展，但仍需要进行大量的优化工作，以确保在所有必要的验证下进行大规模应用。
