Department of Biology, The University of Texas at San Antonio, One UTSA Circle, San Antonio, TX, 78249, USA.
Arch Virol. 2019 Nov;164(11):2823-2828. doi: 10.1007/s00705-019-04385-9. Epub 2019 Sep 4.
A 278-bp region upstream of the beet curly top virus-SpCT (BCTV-SpCT) C2/C3 genes is necessary for promoter activity and exhibits significant sequence similarity to AL2/3 promoter sequences in tomato golden mosaic virus (TGMV). Maximal expression of the downstream C2/3 genes in BCTV-SpCT requires the presence of the C1 protein, which is supported by observations that mutation of the initiator codon for C1 results in decreased C2/C3 expression. This is similar to TGMV and cabbage leaf curl virus, where AL1 is required for maximal AL2/3 expression. Together, these data suggest a common strategy for complementary-sense gene regulation amongst curtoviruses and begomoviruses.
甜菜曲顶病毒-SpCT（BCTV-SpCT）C2/C3 基因上游的 278bp 区域对于启动子活性是必需的，并且与番茄金黄花叶病毒（TGMV）中的 AL2/3 启动子序列具有显著的序列相似性。BCTV-SpCT 下游 C2/C3 基因的最大表达需要 C1 蛋白的存在，这一观察结果得到了支持，即 C1 的起始密码子突变导致 C2/C3 表达降低。这与 TGMV 和甘蓝卷叶病毒相似，其中 AL1 是 AL2/3 表达的最大需要。这些数据共同表明，在负义链病毒和双生病毒之间存在互补性基因调控的共同策略。
