Burke E, Zimmerman S R, Brown D S, Jenke D R
Baxter Healthcare, Inc., William B. Graham Science Center, Round Lake, Illinois 60073.
J Chromatogr Sci. 1988 Oct;26(10):527-32. doi: 10.1093/chromsci/26.10.527.
Matrix interferents are removed from pharmaceutical samples via an on-line, automated column switching process in which the difference in hydrophobicity between the analyte and the interferents allows these species to be isolated in different parts of the chromatographic system. In this case, the interferents are trapped on a cleanup column and are flushed to waste as the analyte undergoes additional separation on an analytical column. The utility of this approach is demonstrated by the quantitation of citrate in pharmaceutical samples by ion suppression, reversed-phase liquid chromatography. The performance of this system is statistically equivalent to that of a manual pretreatment method employing disposable, solid-phase extraction cartridges.
通过在线自动化柱切换过程从药物样品中去除基质干扰物，其中分析物和干扰物之间疏水性的差异使得这些物质能够在色谱系统的不同部分被分离。在这种情况下，干扰物被困在净化柱上，并在分析物在分析柱上进行进一步分离时被冲洗至废液中。通过离子抑制反相液相色谱法定量药物样品中的柠檬酸盐，证明了该方法的实用性。该系统的性能在统计学上等同于采用一次性固相萃取小柱的手动预处理方法。
