Department of Thyroid and Breast Surgery, Bayannur City Hospital, Bayannur, China.
Eur Rev Med Pharmacol Sci. 2019 Aug;23(16):6983-6990. doi: 10.26355/eurrev_201908_18738.
To elucidate the role of miRNA-221-5p in the development of breast cancer (BCa) and its underlying mechanism.
The expression level of miRNA-221-5p in 52 pairs of BCa tissues and adjacent normal tissues was detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). The correlation between miRNA-221-5p expression and pathological indicators of BCa was analyzed. MiRNA-221-5p expression in BCa cells was also determined by qRT-PCR. After transfection of miRNA-221-5p inhibitor in MCF-7 and SKBR3 cells, we detected the regulatory effects of miRNA-221-5p on cellular behaviors through cell counting kit-8 (CCK-8), wound healing, and transwell assay. Finally, the relationship between miRNA-221-5p and E-cadherin in BCa was elucidated.
QRT-PCR results showed that the expression level of miRNA-221-5p in BCa tissues was markedly higher than that in normal tissues. Compared with BCa patients with low expression of miRNA-221-5p, those with high expression had a higher incidence of lymph node metastasis and distant metastasis. However, miRNA-221-5p expression did not correlate with age and sex of BCa patients. MiRNA-221-5p was also highly expressed in BCa cells. Transfection of miRNA-221-5p inhibitor suppressed proliferative, invasive, and migratory potentials of BCa cells. Subsequently, we verified that E-cadherin was lowly expressed in BCa cells, and negatively correlated with miRNA-221-5p. In addition, rescue experiments confirmed that transfection of si-E-cadherin reversed the inhibitory role of miRNA-221-5p knockdown in migratory and invasive potentials of BCa cells.
The expression of miRNA-221-5p remained high in BCa, which was correlated with lymph node metastasis, distant metastasis, and poor prognosis of BCa. MiRNA-221-5p may promote the invasive and migratory potentials of BCa by regulating E-cadherin expression.
阐明 microRNA-221-5p 在乳腺癌（BCa）发生发展中的作用及其机制。
采用实时荧光定量聚合酶链反应（qRT-PCR）检测 52 对 BCa 组织及癌旁正常组织中 microRNA-221-5p 的表达水平，分析 microRNA-221-5p 表达与 BCa 病理指标的相关性。qRT-PCR 检测 BCa 细胞中 microRNA-221-5p 的表达。转染 microRNA-221-5p 抑制剂后，通过细胞计数试剂盒-8（CCK-8）、划痕愈合和 Transwell 实验检测 microRNA-221-5p 对 MCF-7 和 SKBR3 细胞行为的调控作用。最后，阐明 microRNA-221-5p 与 BCa 中 E-钙黏蛋白的关系。
qRT-PCR 结果显示，BCa 组织中 microRNA-221-5p 的表达水平明显高于正常组织。与 microRNA-221-5p 低表达的 BCa 患者相比，高表达的患者淋巴结转移和远处转移的发生率更高。然而，microRNA-221-5p 的表达与 BCa 患者的年龄和性别无关。microRNA-221-5p 在 BCa 细胞中也高表达。转染 microRNA-221-5p 抑制剂可抑制 BCa 细胞的增殖、侵袭和迁移能力。随后，我们验证了 E-钙黏蛋白在 BCa 细胞中低表达，并与 microRNA-221-5p 呈负相关。此外，挽救实验证实，转染 si-E-钙黏蛋白可逆转 microRNA-221-5p 敲低对 BCa 细胞迁移和侵袭能力的抑制作用。
microRNA-221-5p 在 BCa 中呈高表达，与 BCa 的淋巴结转移、远处转移和不良预后相关。microRNA-221-5p 可能通过调节 E-钙黏蛋白的表达促进 BCa 的侵袭和迁移能力。
