LncRNA SNHG7 通过调节 microRNA-186 促进乳腺癌的发展。
LncRNA SNHG7 promotes development of breast cancer by regulating microRNA-186.
机构信息
Department of Breast Surgery, China-Japan Union Hospital, Jilin University, Changchun, China.
出版信息
Eur Rev Med Pharmacol Sci. 2018 Nov;22(22):7788-7797. doi: 10.26355/eurrev_201811_16403.
OBJECTIVE
To elucidate the biological functions of long non-coding RNA (lncRNA) SNHG7 in breast cancer (BC), and its underlying mechanism in the occurrence and progression of BC.
PATIENTS AND METHODS
The expression of SNHG7 in 72 pairs of BC tissues and paracancerous tissues was detected by quantitative Real-time polymerase chain reaction (qRT-PCR). Correlation between SNHG7 expressions with pathological indicators of BC patients was analyzed. Similarly, SNHG7 expression in BC cell lines was determined by qRT-PCR as well. After constructing the small inference RNA of SNHG7, cell proliferation, migration and invasion were determined by cell counting kit-8 (CCK-8), 5-Ethynyl-2'-deoxyuridine (EdU) and transwell assay. MicroRNA-186 expression in BC tissues and cells was accessed. Dual-luciferase reporter gene assay was conducted to verify the binding condition between SNHG7 and microRNA-186.
RESULTS
SNHG7 expression was higher in BC tissues than that of paracancerous tissues. High expression of SNHG7 was positively correlated to tumor stage, lymph node metastasis and distant metastasis, whereas not correlated to age, sex and tumor location of BC. Kaplan-Meier curves revealed that higher expression of SNHG7 is correlated to the worse prognosis of BC patients. SNHG7 was highly expressed in BC cells as well. Knockdown of SNHG7 inhibited proliferative, invasive and migratory abilities of BC cells. QRT-PCR data showed that microRNA-186 is lowly expressed in BC tissues compared with that of paracancerous tissues. MicroRNA-186 was lowly expressed in BC cells as well. Both mRNA and protein levels of microRNA-186 were negatively correlated to SNHG7 in BC tissues. Finally, the dual-luciferase reporter gene assay demonstrated that SNHG7 could be directly targeted by microRNA-186.
CONCLUSIONS
SNHG7 is highly expressed in BC, which is correlated to tumor stage, lymph node metastasis and distant metastasis of BC patients. SNHG7 could promote malignant progression of BC by regulating microRNA-186.
目的
阐明长链非编码 RNA（lncRNA）SNHG7 在乳腺癌（BC）中的生物学功能及其在 BC 发生和发展中的潜在机制。
方法
通过实时定量聚合酶链反应（qRT-PCR）检测 72 对 BC 组织和癌旁组织中 SNHG7 的表达。分析 SNHG7 表达与 BC 患者病理指标的相关性。同样，通过 qRT-PCR 确定 BC 细胞系中 SNHG7 的表达。构建 SNHG7 的小干扰 RNA 后，通过细胞计数试剂盒-8（CCK-8）、5-乙炔基-2'-脱氧尿苷（EdU）和 Transwell 分析测定细胞增殖、迁移和侵袭。检测 BC 组织和细胞中 microRNA-186 的表达。通过双荧光素酶报告基因实验验证 SNHG7 与 microRNA-186 的结合情况。
结果
SNHG7 在 BC 组织中的表达高于癌旁组织。SNHG7 高表达与肿瘤分期、淋巴结转移和远处转移呈正相关，而与 BC 患者的年龄、性别和肿瘤部位无关。Kaplan-Meier 曲线表明，SNHG7 高表达与 BC 患者的预后较差相关。SNHG7 在 BC 细胞中也呈高表达。敲低 SNHG7 抑制 BC 细胞的增殖、侵袭和迁移能力。qRT-PCR 数据显示，与癌旁组织相比，BC 组织中 microRNA-186 的表达较低。BC 细胞中也低表达 microRNA-186。BC 组织中 microRNA-186 的 mRNA 和蛋白水平均与 SNHG7 呈负相关。最后，双荧光素酶报告基因实验表明，SNHG7 可被 microRNA-186 直接靶向。
结论
SNHG7 在 BC 中高表达，与 BC 患者的肿瘤分期、淋巴结转移和远处转移相关。SNHG7 可通过调节 microRNA-186 促进 BC 的恶性进展。
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