Cadet J, Voituriez L, Nardin R, Viari A, Vigny P
Département de Recherche Fondamentale, Centre d'Etudes Nucleáires de Grenoble, France.
J Photochem Photobiol B. 1988 Nov;2(3):321-39. doi: 10.1016/1011-1344(88)85052-8.
The near-UV-induced photoreaction of the bifunctional 8-methoxypsoralen (8-MOP) with 2'-deoxyadenosine (dAdo) was investigated in the dry state. Four main monoadducts of 8-MOP to 2'-deoxyadenosine were separated by high performance liquid chromatography and subsequently characterized by soft ionization mass spectrometry (fast atom bombardment and plasma desorption mass spectrometries) and extensive 1H NMR analysis including nuclear Overhauser effect (NOE) measurements. These new types of furocoumarin-nucleic acid component which appear to be specific to 2'-deoxyadenosine were shown to result from recombination of the 3,4-dihydropyron-4-yl radical of 8-MOP with 2'-deoxyadenosyl radical either at the 1' or the 5' position.
在干燥状态下研究了双功能8-甲氧基补骨脂素（8-MOP）与2'-脱氧腺苷（dAdo）的近紫外诱导光反应。通过高效液相色谱法分离出8-MOP与2'-脱氧腺苷的四种主要单加合物，随后通过软电离质谱（快原子轰击和等离子体解吸质谱）以及包括核Overhauser效应（NOE）测量在内的广泛的1H NMR分析对其进行表征。这些似乎是2'-脱氧腺苷特有的新型呋喃香豆素-核酸成分被证明是由8-MOP的3,4-二氢吡喃-4-基自由基与2'-脱氧腺苷自由基在1'或5'位置重组产生的。
