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克隆的海豚线粒体基因组的分子特征

Molecular characterization of a cloned dolphin mitochondrial genome.

作者信息

Southern S O, Southern P J, Dizon A E

机构信息

Scripps Institution of Oceanography, Marine Biology Research Division, University of California, San Diego, La Jolla 92093.

出版信息

J Mol Evol. 1988;28(1-2):32-42. doi: 10.1007/BF02143495.

DOI:10.1007/BF02143495
PMID:3148740
Abstract

DNA clones have been isolated that span the complete mitochondrial (mt) genome of the dolphin, Cephalorhynchus commersonii. Hybridization experiments with purified primate mtDNA probes have established that there is close resemblance in the general organization of the dolphin mt genome and the terrestrial mammalian mt genomes. Sequences covering 2381 bp of the dolphin mt genome from the major noncoding region, three tRNA genes, and parts of the genes encoding cytochrome b, NADH dehydrogenase subunit 3 (ND3), and 16S rRNA have been compared with corresponding regions from other mammalian genomes. There is a general tendency throughout the sequenced regions for greater similarity between dolphin and bovine mt genomes than between dolphin and rodent or human mt genomes.

摘要

已经分离出跨越海豚(小头鼠海豚)完整线粒体(mt)基因组的DNA克隆。用纯化的灵长类动物线粒体DNA探针进行的杂交实验表明,海豚线粒体基因组的总体组织与陆生哺乳动物的线粒体基因组非常相似。已将来自主要非编码区、三个tRNA基因以及编码细胞色素b、NADH脱氢酶亚基3(ND3)和16S rRNA的部分基因的2381 bp海豚线粒体基因组序列与其他哺乳动物基因组的相应区域进行了比较。在整个测序区域中,一般趋势是海豚和牛的线粒体基因组之间的相似性高于海豚与啮齿动物或人类线粒体基因组之间的相似性。

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