Cedergren R, Gray M W, Abel Y, Sankoff D
Department de biochimie, Université de Montréal, Québec, Canada.
J Mol Evol. 1988;28(1-2):98-112. doi: 10.1007/BF02143501.
Sequences of small subunit (SSU) and large subunit (LSU) ribosomal RNA genes from archaebacteria, eubacteria, and the nucleus, chloroplasts, and mitochondria of eukaryotes have been compared in order to identify the most conservative positions. Aligned sets of these positions for both SSU and LSU rRNA have been used to generate tree diagrams relating the source organisms/organelles. Branching patterns were evaluated using the statistical bootstrapping technique. The resulting SSU and LSU trees are remarkably congruent and show a high degree of similarity with those based on alternative data sets and/or generated by different techniques. In addition to providing insights into the evolution of prokaryotic and eukaryotic (nuclear) lineages, the analysis reported here provides, for the first time, an extensive phylogeny of the mitochondrial lineage.
为了确定最保守的位置，对古细菌、真细菌以及真核生物的细胞核、叶绿体和线粒体的小亚基（SSU）和大亚基（LSU）核糖体RNA基因序列进行了比较。已使用这些SSU和LSU rRNA的对齐位置集来生成与源生物/细胞器相关的树形图。使用统计自展技术评估分支模式。所得的SSU和LSU树非常一致，并且与基于替代数据集和/或由不同技术生成的树具有高度相似性。除了深入了解原核生物和真核生物（核）谱系的进化外，本文报道的分析首次提供了线粒体谱系的广泛系统发育。
