Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia.
Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia,
Caries Res. 2020;54(1):36-42. doi: 10.1159/000502337. Epub 2019 Sep 5.
Accumulated intra-lesion protein such as serum albumin has been speculated to impede remineralisation of carious enamel lesions. The aim of this study was to assess whether intra-lesion bovine serum albumin (BSA) affected subsequent remineralisation of enamel subsurface lesions. Confocal microscopy was used to confirm localisation of BSA in artificial enamel subsurface lesions and its subsequent degradation by a high pH sodium hypochlorite treatment. An in vitro remineralisation experiment tested the effect of intra-lesion BSA, and its degradation by sodium hypochlorite, on remineralisation of subsurface lesions by casein phosphopeptide stabilised amorphous calcium fluoride phosphate. In addition, lesions without BSA were pre-treated with one of 2 high pH solutions (sodium hypochlorite or sodium hydroxide) prior to remineralisation to test whether the high pH pre-treatment influenced remineralisation. Data were obtained on remineralisation using transverse microradiography and were analysed with a one-way ANOVA. Intra-lesion BSA had no significant effect on remineralisation compared with that of control lesions. Pre-treatment of BSA-containing lesions with sodium hypochlorite significantly increased remineralisation. The lesions without BSA that were pre-treated with either sodium hypochlorite or sodium hydroxide also showed the same level of remineralisation as the BSA-containing lesions pre-treated with sodium hypochlorite indicating that the increased remineralisation was pH related. Hence, it was concluded that intra-lesion BSA did not affect remineralisation of artificial enamel subsurface lesions in this model system and that a high pH pre-treatment enhanced remineralisation.
已推测病灶内蓄积的蛋白质（如血清白蛋白）会阻碍龋损牙釉质的再矿化。本研究旨在评估病灶内牛血清白蛋白（BSA）是否会影响牙釉质下病变的后续再矿化。共聚焦显微镜用于确认 BSA 在人工牙釉质下病变中的定位及其随后被高 pH 次氯酸钠处理降解。体外再矿化实验测试了病灶内 BSA 及其被次氯酸钠降解对酪蛋白磷酸肽稳定无定形氟化钙磷再矿化牙釉质下病变的影响。此外，在再矿化之前，将不含 BSA 的病变用 2 种高 pH 溶液（次氯酸钠或氢氧化钠）之一进行预处理，以测试高 pH 预处理是否会影响再矿化。使用横向显微放射摄影术获得再矿化数据，并使用单向方差分析进行分析。与对照病变相比，病灶内 BSA 对再矿化无显著影响。用次氯酸钠预处理含 BSA 的病变可显著增加再矿化。用次氯酸钠或氢氧化钠预处理不含 BSA 的病变也显示出与用次氯酸钠预处理的含 BSA 病变相同的再矿化水平，这表明增加的再矿化与 pH 有关。因此，结论是病灶内 BSA 不会影响该模型系统中人工牙釉质下病变的再矿化，高 pH 预处理可增强再矿化。
