Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia.
Oral Health Cooperative Research Centre, Melbourne Dental School, Bio21 Institute, The University of Melbourne, Melbourne, Victoria, Australia,
Caries Res. 2021;55(2):130-136. doi: 10.1159/000513609. Epub 2021 Feb 1.
Remineralisation of demineralised enamel subsurface lesions can be enhanced by pretreatment of the lesions with base (NaOH). The aim of this study was to test the effect of intralesion pH modulation on remineralisation of demineralised enamel subsurface lesions by casein phosphopeptide-stabilised amorphous calcium fluoride phosphate (CPP-ACFP) in vitro. Two remineralisation models were utilised, the first involving 60-min cyclic pH modulation for 105 h and the second involved short-term cyclic pH modulation (12-min cycle, 240 min total duration) compared with the equivalent time of continuous treatment (200 min total duration). The intralesion pH modulation was achieved by cyclic exposure to a pH 12.9 NaOH solution and a CPP-ACFP remineralisation solution at pH 5.5. Percent remineralisation was assessed using transverse microradiography with data statistically analysed using a 2-sample Student t test. For the first model, the intralesion pH modulation group had significantly (p < 0.001) higher remineralisation (43.8 ± 6.9%) than the control group (28.2 ± 5.8%) cycled with water. For the second model, the intralesion pH modulation group had significantly (p < 0.001) higher remineralisation (23.1 ± 3.4%) than the group with continuous equivalent CPP-ACFP treatment time (1.9 ± 1.3%). In both models, intralesion pH modulation significantly accelerated remineralisation, and this was attributed to the effect pH modulation had on the diffusion gradients of ions/ion pairs and the degree of saturation with respect to apatite phases within the lesion fluid.
脱矿釉质表面下病变的再矿化可以通过用碱（NaOH）预处理病变来增强。本研究的目的是测试通过酪蛋白磷酸肽稳定的无定形氟化钙磷（CPP-ACFP）对釉质表面下病变的脱矿进行再矿化时，病变内 pH 值调节对再矿化的影响。本研究使用了两种再矿化模型，第一种涉及 60 分钟的 pH 值循环调节，持续 105 小时，第二种涉及短期的 pH 值循环调节（12 分钟周期，总持续时间 240 分钟），与连续治疗（总持续时间 200 分钟）等效。通过周期性暴露于 pH 值为 12.9 的 NaOH 溶液和 pH 值为 5.5 的 CPP-ACFP 再矿化溶液来实现病变内 pH 值调节。使用横向显微放射摄影术评估再矿化百分比，并使用双样本学生 t 检验对数据进行统计学分析。对于第一个模型，病变内 pH 值调节组的再矿化率（43.8±6.9%）显著高于对照组（28.2±5.8%），对照组在水介质中循环。对于第二个模型，病变内 pH 值调节组的再矿化率（23.1±3.4%）显著高于具有连续等效 CPP-ACFP 处理时间的组（1.9±1.3%）。在这两种模型中，病变内 pH 值调节都显著加速了再矿化，这归因于 pH 值调节对病变内离子/离子对扩散梯度和与病变内磷灰石相饱和度的影响。
