Visser Erik A, Wegrzyn Jill L, Steenkamp Emma T, Myburg Alexander A, Naidoo Sanushka
Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute (FABI), Centre for Bioinformatics and Computational Biology, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa.
Department of Ecology and Evolutionary Biology, University of Connecticut, Storrs, CT 06269, USA.
Microorganisms. 2019 Sep 4;7(9):315. doi: 10.3390/microorganisms7090315.
poses a serious threat to many pine species in both commercial and natural pine forests. Knowledge regarding the molecular basis of pine- host-pathogen interactions could assist efforts to produce more resistant planting stock. This study aimed to identify molecular responses underlying resistance against . A dual RNA-seq approach was used to investigate host and pathogen expression in challenged (resistant) and (susceptible), at three- and seven-days post inoculation. RNA-seq reads were mapped to combined host-pathogen references for both pine species to identify differentially expressed genes (DEGs). genes expressed during infection showed decreased ergosterol biosynthesis in relative to . For , enriched gene ontologies and DEGs indicated roles for auxin-, ethylene-, jasmonate- and salicylate-mediated phytohormone signalling. Correspondingly, key phytohormone signaling components were down-regulated in . Key ergosterol biosynthesis genes were expressed at lower levels during infection of the resistant relative to the susceptible host. This study further suggests that coordination of phytohormone signaling is required for resistance in , while a comparatively delayed response and impaired phytohormone signaling contributes to susceptibility in .
对商业松林和天然松林中的许多松树品种都构成了严重威胁。关于松树-宿主-病原体相互作用的分子基础的知识有助于培育更具抗性的苗木。本研究旨在确定抗御……的分子反应。采用双RNA测序方法，在接种后三天和七天，研究了受挑战的（抗性）和（敏感）……中宿主和病原体的表达情况。将RNA测序读数映射到两种松树的宿主-病原体组合参考序列上，以鉴定差异表达基因（DEG）。感染期间表达的……基因相对于……显示麦角固醇生物合成减少。对于……，富集的基因本体和差异表达基因表明生长素、乙烯、茉莉酸和水杨酸介导的植物激素信号传导发挥了作用。相应地，关键植物激素信号传导成分在……中下调。在抗性宿主感染期间，关键的麦角固醇生物合成基因表达水平低于敏感宿主。本研究进一步表明，植物激素信号传导的协调是……产生抗性所必需的，而相对延迟的反应和受损的植物激素信号传导导致……易感性增加。
