Benhalima Lamia, Amri Sandra, Bensouilah Mourad, Ouzrout Rachid
Dr. Lamia Benhalima, Assistant Professor, Department of Biology, Universite 8 Mai 1945, Guelma, Algeria.
Dr. Sandra Amri, Assistant Professor, Department of Biology, Universite 8 Mai 1945, Guelma, Algeria.
Pak J Med Sci. 2019 Sep-Oct;35(5):1322-1328. doi: 10.12669/pjms.35.5.336.
With the emergence of antibiotic resistance and the hospital acquired infection, the interest for antimicrobial agents has recently increased again in public health. Copper is recommended as a supplementary method of increasing biological safety in the hospital environment. The objective of this study was to determine the antibacterial activity of copper sulfate salts on strains of bacterial pathogens isolated from different clinical pictures in different health establishment in Algeria.
A total of 25 different bacterial isolates (16 , 5 , and 4 were tested for susceptibility to copper sulfate using minimum inhibitory concentration (MIC-Cu) and minimum bactericidal concentrations (MBC-Cu) determinations. All isolates were also tested for susceptibility to six antibiotics.
Antibiotic susceptibility studies revealed that 100% of isolates were resistant to one or more antibiotics. Fifty two percent of isolates were very susceptible to copper sulfate, with MICs ranging from 100 to 200 µg/ml. MBC-Cu = 1600 μg/ml showed the best bactericidal effect against the great majority of studied bacteria (52%). A good bactericidal activities of copper sulfate were recorded against and (MBC/MIC=1). The Gram-negative bacteria isolates which were copper resistant also showed a high resistance to chloramphenicol (=0.78) and Trimethoprime (=0.61). Furthermore, the strains that were no-susceptible to three different antimicrobial classes (, ) were not resistant to copper sulfate.
Copper sulfate salts has significant antibacterial activity against multi-drug resistant nosocomial pathogens.
随着抗生素耐药性的出现以及医院获得性感染的发生，抗菌药物在公共卫生领域的关注度最近再次提高。铜被推荐作为提高医院环境生物安全性的一种补充方法。本研究的目的是确定硫酸铜盐对从阿尔及利亚不同医疗机构不同临床病例中分离出的细菌病原体菌株的抗菌活性。
使用最低抑菌浓度（MIC-Cu）和最低杀菌浓度（MBC-Cu）测定法，对总共25种不同的细菌分离株（16种、5种和4种）进行硫酸铜敏感性测试。所有分离株还进行了对六种抗生素的敏感性测试。
抗生素敏感性研究表明，100%的分离株对一种或多种抗生素耐药。52%的分离株对硫酸铜非常敏感，MIC范围为100至200μg/ml。MBC-Cu = 1600μg/ml对绝大多数研究细菌（52%）显示出最佳杀菌效果。硫酸铜对[具体细菌名称1]和[具体细菌名称2]具有良好的杀菌活性（MBC/MIC = 1）。对铜耐药的革兰氏阴性菌分离株对氯霉素（= 0.78）和甲氧苄啶（= 0.61）也表现出高耐药性。此外，如果菌株对三种不同抗菌类别（[具体抗菌类别1]、[具体抗菌类别2]）不敏感，则对硫酸铜不耐药。
硫酸铜盐对多重耐药的医院病原体具有显著的抗菌活性。
