Mansoor Afsheen, Mehmood Mazhar, Ishtiaq Muhammad, Jamal Asif
Afsheen Mansoor, Associate Professor, School of Dentistry, Shaheed Zulfiqar Ali Bhutto, Medical University, Islamabad, Pakistan. Quaid-i-Azam University, Islamabad, Pakistan.
Mazhar Mehmood Professor, Pakistan Institute of Engineering and Applied, Sciences, Islamabad, Pakistan.
Pak J Med Sci. 2023 Sep-Oct;39(5):1249-1254. doi: 10.12669/pjms.39.5.7851.
To evaluate antagonistic role of titanium oxide nanoparticles against selected dental caries promoting bacteria.
This in vitro-experimental study was conducted at Pakistan Institute of Engineering and Applied Sciences (PIEAS), National Institute of Health (NIH) and School of Dentistry (SOD), Islamabad for the period of one year from February 2022 to January 2023. Modified hydrothermal heating method was used to prepare titanium oxide nanoparticles (TiONps). Size, shape, phase, band gap energy, surface and elemental composition of Nps were deciphered by application of various modern techniques including x-ray diffraction spectroscopy (XRD), scanning electron microscopy (SEM), dynamic light scattering (DLS), UV-Vis diffuse reflectance spectroscopy (DRS), atomic force microscopy (AFM), energy dispersive x-ray spectroscopy (EDX). Antimicrobial action of nanoparticles was evaluated against representatives of gram-positive (mono-derm) and Gram negative bacteria (di-derm) responsible for promoting dental caries. The zones of inhibition were calculated by disc diffusion method for each bacterial strain.
Characterization revealed that TiONps were having an average size of 54nm, showing anatase-rutile phase having spherical, with very few- irregularly shaped particles. TiONps contained only pure titanium and oxygen in the EDX image but organic compounds in FTIR scan. Results of antimicrobial action indicated their potent bactericidal action against while comparatively less activity against ..
TiONps fabricated by modified protocol displayed an effective antimicrobial activity and can be used as an alternative to the contemporary chemotherapeutics against selected bacterial pathogens to prevent dental caries.
评估纳米二氧化钛对某些致龋菌的拮抗作用。
本体外实验研究于2022年2月至2023年1月在巴基斯坦工程与应用科学研究所（PIEAS）、国立卫生研究院（NIH）和伊斯兰堡牙科学院（SOD）进行，为期一年。采用改良水热加热法制备纳米二氧化钛（TiONps）。通过应用各种现代技术，包括X射线衍射光谱（XRD）、扫描电子显微镜（SEM）、动态光散射（DLS）、紫外可见漫反射光谱（DRS）、原子力显微镜（AFM）、能量色散X射线光谱（EDX），来解析纳米颗粒的尺寸、形状、相、带隙能量、表面和元素组成。评估纳米颗粒对导致龋齿的革兰氏阳性（单皮层）和革兰氏阴性细菌（双皮层）代表菌的抗菌作用。通过纸片扩散法计算每种细菌菌株的抑菌圈。
表征显示，TiONps的平均尺寸为54nm，呈现锐钛矿-金红石相，呈球形，只有极少数形状不规则的颗粒。EDX图像显示TiONps仅含有纯钛和氧，但傅里叶变换红外光谱扫描显示含有有机化合物。抗菌作用结果表明，它们对……具有强大的杀菌作用，而对……的活性相对较低。
通过改良方案制备的TiONps显示出有效的抗菌活性，可作为当代化学疗法的替代品，用于对抗某些细菌病原体以预防龋齿。
