Zha Xu, Wu Guojiu, Zhang Hong, Yang Yanni, Zhang Yuanping, Ma Linkun
Department of the Ophthalmology, Second Affiliated Hospital of Kunming Medical University, Kunming, People's Republic of China.
Anim Cells Syst (Seoul). 2019 Apr 11;23(4):241-245. doi: 10.1080/19768354.2019.1592021. eCollection 2019.
Hereditary retinal disease (HRD) is the primary retinal degeneration that leads to severe visual impairments and refractory blindness, and the therapy of HRD was most important in ophthalmology. The apoptosis of retinal cells plays important roles in HRD progression. Therefore, in this study, we explore the mechanism of H2O2 and blue light-induced apoptosis of ARPE-19 cells. Co-immunoprecipitation (Co-IP) is employed to test the interactions between proteins, and western blotting is used to detect the protein levels. Apoptosis is analyzed by Flow cytometry. Our results found that PRDX6 could interact with RARA in ARPE-19 cells, and H2O2 and blue light could significantly reduce the RARA protein expression, and also could inhibit the interaction between PRDX6 and RARA. Using a rescue experiment, we further elucidated that H2O2 and blue light reduced the RARA expression via down-regulating PRDX6. And H2O2 and blue light induced the ARPE-19 cell apoptosis via decreasing the expression of PRDX6. Our results suggested that the interaction between PRDX6 and RARA played important roles in the apoptosis of ARPE-19 cells.
遗传性视网膜疾病（HRD）是导致严重视力损害和难治性失明的主要视网膜变性疾病，HRD的治疗在眼科领域至关重要。视网膜细胞凋亡在HRD进展中起重要作用。因此，在本研究中，我们探讨了H2O2和蓝光诱导ARPE-19细胞凋亡的机制。采用免疫共沉淀（Co-IP）检测蛋白质之间的相互作用，并用蛋白质印迹法检测蛋白质水平。通过流式细胞术分析细胞凋亡情况。我们的结果发现，PRDX6可与ARPE-19细胞中的RARA相互作用，H2O2和蓝光可显著降低RARA蛋白表达，还可抑制PRDX6与RARA之间的相互作用。通过拯救实验，我们进一步阐明，H2O2和蓝光通过下调PRDX6降低RARA表达。并且H2O2和蓝光通过降低PRDX6的表达诱导ARPE-19细胞凋亡。我们的结果表明，PRDX6与RARA之间的相互作用在ARPE-19细胞凋亡中起重要作用。
