anoctamin-1影响间变性甲状腺癌细胞的迁移和侵袭。

Kim Jae-Young, Youn Hwa Young, Choi June, Baek Seung Kuk, Kwon Soon Young, Eun Bok Kee, Park Jae-Yong, Oh Kyoung Ho

Department of Veterinary Internal Medicine, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.

Department of Otorhinolaryngology-Head and Neck Surgery, Korea University College of Medicine, Seoul, Republic of Korea.

Anim Cells Syst (Seoul). 2019 May 10;23(4):294-301. doi: 10.1080/19768354.2019.1614981. eCollection 2019.

Anaplastic thyroid carcinoma (ATC) is a rare malignancy with very poor prognosis. The exact cause underlying its strong aggressive nature is not clear. Here, we discovered the elevated expression of anoctamin-1 (ANO1; Ca-activated Cl channels) in advanced-stage ATC tissue. Using different ATC cell lines, the degree of expression of ANO1 was found to be related to the degree of ATC cell invasion by quantitative reverse transcription polymerase chain reaction and western blotting. Suppression of ANO1 activity either by selective inhibitor (T16Ainh-A01) or by siRNA significantly attenuated the migration and invasion of ATC cells. In conclusion, ANO1 appears to increase the ability of ATC cells to invade and migrate. Our results also suggest that the expression of ANO1 in patients with ATC may be helpful in predicting the prognosis of ATC.

间变性甲状腺癌（ATC）是一种预后极差的罕见恶性肿瘤。其强烈侵袭性的确切病因尚不清楚。在此，我们发现晚期ATC组织中anoctamin-1（ANO1；钙激活氯离子通道）表达升高。通过定量逆转录聚合酶链反应和蛋白质免疫印迹法，在不同的ATC细胞系中发现ANO1的表达程度与ATC细胞侵袭程度相关。通过选择性抑制剂（T16Ainh-A01）或小干扰RNA抑制ANO1活性可显著减弱ATC细胞的迁移和侵袭。总之，ANO1似乎增强了ATC细胞的侵袭和迁移能力。我们的结果还表明，ATC患者中ANO1的表达可能有助于预测ATC的预后。