School of Food & Pharmaceutical Engineering, Zhaoqing University, Zhaoqing, P. R. China.
College of Environmental and Chemical Engineering, Zhaoqing University, Zhaoqing, P. R. China.
J Sep Sci. 2019 Nov;42(21):3372-3381. doi: 10.1002/jssc.201900576. Epub 2019 Sep 18.
A novel magnetic molecularly imprinted polymer adsorbing material was successfully synthesized to detect ribavirin in animal feedstuff. Molecularly imprinted polymer was prepared through surface polymerization by using ribavirin as template molecule, methyl methacrylate, and γ-methacryloxypropyl trimethoxy silane functionalized magnetic mesoporous silica as bifunctional monomers, and ethylene diglycidyl ether as crosslinking agent. The prepared magnetic molecularly imprinted polymer was characterized by scanning electron microscopy and infrared spectroscopy. Static and dynamic adsorption experiments and selective adsorption analysis were performed to evaluate the adsorption and selectivity of magnetic molecularly imprinted polymer. Different experiments were conducted to optimize the magnetic solid-phase extraction conditions. Under optimal experimental conditions, a magnetic molecularly imprinted solid-phase extraction coupled with high-performance liquid chromatography method was successfully developed for ribavirin detection. The established method achieved a satisfactory linear range of 0.20-50 mg/L (R > 0.99) and a low detection limit (0.081 mg/kg). An average recovery of 92-105% with relative standard deviation of <6.5% was obtained upon the application of the developed method to detect ribavirin in real feedstuff samples. Thus, established method can be used for the rapid and simple separation and detection of added ribavirin in feedstuff.
一种新型的磁性分子印迹聚合物吸附材料被成功合成，用于检测动物饲料中的利巴韦林。通过表面聚合，以利巴韦林为模板分子、甲基丙烯酸甲酯和γ-甲基丙烯酰氧基丙基三甲氧基硅烷功能化磁性介孔硅作为双功能单体、以及乙二醇二缩水甘油醚作为交联剂，制备了分子印迹聚合物。通过扫描电子显微镜和红外光谱对制备的磁性分子印迹聚合物进行了表征。通过静态和动态吸附实验以及选择性吸附分析，评价了磁性分子印迹聚合物的吸附和选择性。进行了不同的实验以优化磁性固相萃取条件。在最佳实验条件下，成功开发了一种基于高效液相色谱法的磁性分子印迹固相萃取法用于利巴韦林的检测。该方法在 0.20-50 mg/L 范围内（R > 0.99）具有令人满意的线性关系，检测限为 0.081 mg/kg。该方法应用于实际饲料样品中添加利巴韦林的检测，平均回收率为 92-105%，相对标准偏差 <6.5%。因此，该方法可用于饲料中添加利巴韦林的快速简便分离和检测。
