P1G10，一种来源于 的蛋白水解片段，可刺激急性紫外线 B 辐射暴露后的组织修复。
P1G10, the Proteolytic Fraction from , Stimulates Tissue Repair after Acute Exposure to Ultraviolet B Radiation.
机构信息
Departamento de Farmacologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av Antônio Carlos 6627, Belo Horizonte 31270-901, MG, Brazil.
Faculdade de Medicina do Mucuri, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Rua do Cruzeiro, nº 01, Bairro Jardim São Paulo, Teófilo Otoni 39803-371, MG, Brazil.
出版信息
Int J Mol Sci. 2019 Sep 6;20(18):4373. doi: 10.3390/ijms20184373.
BACKGROUND
P1G10 is a cysteine proteolytic fraction from latex, obtained by chromatographic separation on Sephadex-G10 and ultrafiltration. This fraction enhances healing in different models of skin lesions, and displays a protective/healing effect against gastric ulcers, where it was suggested an antioxidant role.
METHODS
We evaluated here the effect of topical treatment with P1G10, in mice lesions induced by UVB.
RESULTS
After single exposure to 2.4 J cm UVB, P1G10 reduced erythema, increased cellularity of hypodermis, enhanced MPO activity and IL1β, and inhibited COX levels. These results point to an anti-inflammatory effect by P1G10. This fraction displayed antioxidant activity by reversing the depletion of glutathione (GSH), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and reducing the catalase activity increased by UVB. These changes may be related to a reduction in MDA observed in groups treated with P1G10. P1G10 also inhibited MMP-9, caspase-3 and pkat while increasing p53 levels.
背景
P1G10 是从乳胶中分离得到的半胱氨酸蛋白水解物，通过 Sephadex-G10 色谱分离和超滤获得。该部分在不同的皮肤损伤模型中增强愈合，并显示出对胃溃疡的保护/愈合作用，其中提示具有抗氧化作用。
方法
我们在这里评估了 P1G10 对 UVB 诱导的小鼠损伤的局部治疗效果。
结果
单次暴露于 2.4 J cm 的 UVB 后，P1G10 可减少红斑，增加真皮细胞的数量，增加 MPO 活性和 IL1β，并抑制 COX 水平。这些结果表明 P1G10 具有抗炎作用。该部分通过逆转 UVB 引起的谷胱甘肽 (GSH)、谷胱甘肽过氧化物酶 (GSH-Px)、超氧化物歧化酶 (SOD) 的消耗和减少增加的过氧化氢酶活性显示出抗氧化活性。这些变化可能与用 P1G10 处理的组中观察到的 MDA 减少有关。P1G10 还抑制 MMP-9、caspase-3 和 pkat，同时增加 p53 水平。
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