Departamento de Ingeniería en Alimentos, Universidad de La Serena, La Serena, Chile; Laboratorio de Bioquímica y Biología Molecular, Departamento de Biología, Universidad de La Serena, La Serena, Chile; Millennium Nucleus for Fungal Integrative and Synthetic Biology (FISB), Chile.
Departamento de Ingeniería en Alimentos, Universidad de La Serena, La Serena, Chile.
Int J Food Microbiol. 2019 Jan 16;289:7-16. doi: 10.1016/j.ijfoodmicro.2018.08.027. Epub 2018 Aug 28.
The aim of this study was to determine the antifungal activity of the proteolytic fraction P1G10 from Vasconcellea cundinamarcencis (ex-Carica candamarcensis) against Botrytis cinerea, the causative agent of pre- and postharvest damaging disease in fruit and vegetables. The survival of B. cinerea at different concentrations of P1G10 showed that 1 mg/mL inhibited 50% of mycelium growth after 72 h incubation. The kinetic of growth inhibition fits the Weibull distribution function, and the data was confirmed by the IC survival assay. The study shows that P1G10 inhibits conidia germination and germ tube elongation of B. cinerea relative to untreated conidia. Hypersensitivity to cell wall-perturbing agents (Calcofluor white and Congo red) was observed in mycelium cells treated with P1G10. In addition, P1G10 exhibited inhibitory effect on the adhesion of conidia, provoked alterations in membrane integrity and induced production of reactive oxygen species accompanied by cellular damage. Our results highlight the effect of P1G10 on mycelium growth, cell wall alterations, membrane integrity and adhesion. P1G10 emerges as promising antifungal to control disease causing agents in the food agroindustry.
本研究旨在确定 Vasconcellea cundinamarcencis（前 Carica candamarcensis）的蛋白水解部分 P1G10 对灰葡萄孢（Botrytis cinerea）的抗真菌活性，灰葡萄孢是水果和蔬菜采前和产后破坏性疾病的病原体。不同浓度的 P1G10 对 B. cinerea 的生存能力的影响表明，在 72 h 孵育后，1mg/mL 的 P1G10 抑制了 50%的菌丝生长。生长抑制的动力学符合 Weibull 分布函数，IC 生存测定证实了这一点。研究表明，与未处理的分生孢子相比，P1G10 抑制了 B. cinerea 的分生孢子萌发和芽管伸长。用 P1G10 处理的菌丝细胞对细胞壁破坏剂（Calcofluor white 和 Congo red）表现出超敏反应。此外，P1G10 对附着的分生孢子表现出抑制作用，引起细胞壁完整性改变，并诱导活性氧物质的产生，导致细胞损伤。我们的结果强调了 P1G10 对菌丝生长、细胞壁改变、膜完整性和附着的影响。P1G10 有望成为控制食品农业产业中致病因子的抗真菌剂。
