Chen Chin-Chia, Lin Chi, Chen Min-Hung, Chiang Po-Yuan
Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan.
Agriculture & Food Agency Council of Agriculture Executive Yuan Marketing & Processing Division, Taipei 10050, Taiwan.
Foods. 2019 Sep 6;8(9):393. doi: 10.3390/foods8090393.
Because of the high nutritional value of anthocyanin in purple sweet potatoes (TN57), the stability and quality of anthocyanin in purple sweet potatoes during and after the processing were investigated in this study. First of all, the extraction methods with different parameters, such as temperature, time, solid-liquid ratio, pH value, and solvent were employed to get better extraction efficiencies. After that, DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis (3-ethylbenz thiazoline-6-sulphonic acid) were used to evaluate the anti-oxidation ability of the extracts. Lastly, the thermal degradation kinetics and photodegradation were used to obtain important degradation factors including the content of anthocyanin residue, degradation index (DI), color parameters, reaction rate (k), and chromatic aberration (ΔE). We found that the extraction condition as using 60% ethanol with 1% citric acid under 80 °C for 40 min was optimal for purple sweet potatoes, which obtained antioxidant capacity as 333 μM TE DPPH, 376 μM TE ABTS, and 593.6 μM TE ferric ion reducing antioxidant power (FRAP). The results indicate that the most important parameter for the stability of anthocyanin in the purple sweet potato extract (PSPAE) was the pH value rather than temperature. In addition, there was no significant difference in chromatic aberration between the light and dark storage conditions under 37 °C. Thus, PSPAE has the potential to be developed as health foods and drinks rich in anthocyanin.
由于紫甘薯(TN57)中花青素具有较高的营养价值,本研究对紫甘薯花青素在加工过程中和加工后的稳定性及品质进行了研究。首先,采用不同参数的提取方法,如温度、时间、固液比、pH值和溶剂,以获得更好的提取效率。之后,使用DPPH(1,1-二苯基-2-苦基肼)和ABTS(2,2'-联氮-双-(3-乙基苯并噻唑啉-6-磺酸)来评估提取物的抗氧化能力。最后,利用热降解动力学和光降解来获得重要的降解因子,包括花青素残留量、降解指数(DI)、颜色参数、反应速率(k)和色差(ΔE)。我们发现,对于紫甘薯来说,最佳提取条件是在80℃下使用含1%柠檬酸的60%乙醇提取40分钟,该条件下获得的抗氧化能力为333μM TE DPPH、376μM TE ABTS和593.6μM TE铁离子还原抗氧化能力(FRAP)。结果表明,紫甘薯提取物(PSPAE)中花青素稳定性的最重要参数是pH值而非温度。此外,在37℃下,光照和黑暗储存条件下的色差没有显著差异。因此,PSPAE有潜力被开发成富含花青素的健康食品和饮料。