Department of Neurology, Xiangya Hospital, Central South University, 87 Xiangya Road, Changsha, Hunan 410008, People's Republic of China.
Center for Drug Delivery System, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
J Synchrotron Radiat. 2019 Sep 1;26(Pt 5):1742-1750. doi: 10.1107/S160057751900674X. Epub 2019 Jul 12.
Thorough investigation of the three-dimensional (3D) configuration of the vasculature of mouse brain remains technologically difficult because of its complex anatomical structure. In this study, a systematic analysis is developed to visualize the 3D angioarchitecture of mouse brain at ultrahigh resolution using synchrotron-radiation-based propagation phase-contrast imaging. This method provides detailed restoration of the intricate brain microvascular network in a precise 3D manner. In addition to depicting the delicate 3D arrangements of the vascular network, 3D virtual micro-endoscopy is also innovatively performed to visualize randomly a selected vessel within the brain for both external 3D micro-imaging and endoscopic visualization of any targeted microvessels, which improves the understanding of the intrinsic properties of the mouse brain angioarchitecture. Based on these data, hierarchical visualization has been established and a systematic assessment on the 3D configuration of the mouse brain microvascular network has been achieved at high resolution which will aid in advancing the understanding of the role of vasculature in the perspective of structure and function in depth. This holds great promise for wider application in various models of neurovascular diseases.
由于其复杂的解剖结构，对小鼠脑血管的三维（3D）结构进行彻底研究在技术上仍然具有挑战性。在这项研究中，我们开发了一种系统分析方法，使用基于同步辐射的传播相位对比成像以超高分辨率可视化小鼠脑的 3D 血管结构。该方法以精确的 3D 方式提供了复杂脑微血管网络的详细重建。除了描绘血管网络的精细 3D 排列外，还创新性地进行了 3D 虚拟微血管内窥镜检查，以在脑内随机选择一个选定的血管进行可视化，从而实现外部 3D 微观成像和任何靶向微血管的内窥镜可视化，这有助于深入了解小鼠脑血管结构的内在特性。基于这些数据，已经建立了分层可视化，并以高分辨率实现了对小鼠脑微血管网络 3D 结构的系统评估，这将有助于从结构和功能的角度深入了解血管在其中的作用。这在神经血管疾病的各种模型中具有广泛的应用前景。
