Institute of Biomaterials and Bioengineering, Tokyo Medical and Dental University, 2-3-10 Kanda-surugadai, Chiyoda, Tokyo 101-0062, Japan.
J Mater Chem B. 2019 Nov 21;7(43):6655-6669. doi: 10.1039/c9tb00718k. Epub 2019 Sep 6.
Solid-state electrochemical sensors are developing as a new platform for liquid biopsy, combining detection and analysis of nucleic acids with isothermal nucleic acid amplification reactions. For the detection of small nucleic acids such as cfDNA, ctDNA and miRNA in liquid biopsy, further development of highly sensitive and precise devices is required to transcend the barrier of classical detection technology. Electrochemical sensors based on engineering approaches are advantageous for miniaturization of systems and convenience of diagnosis, and the analysis of data obtained with these sensors dovetails with transition to an information society. This review briefly surveys the current development of electrochemical sensors combined with isothermal amplification for nucleic acid detection and predicts the future direction of research.
固态电化学传感器作为液体活检的新平台正在发展，将核酸的检测和分析与等温核酸扩增反应相结合。对于液体活检中 cfDNA、ctDNA 和 miRNA 等小核酸的检测，需要进一步开发高灵敏度和精确的设备来超越经典检测技术的障碍。基于工程方法的电化学传感器有利于系统的小型化和诊断的便利性，并且对这些传感器获得的数据进行分析符合向信息社会的转变。本文简要综述了与等温扩增相结合的用于核酸检测的电化学传感器的最新发展，并预测了研究的未来方向。
