Department of Anatomy, Division Surgical Specialties, University Medical Center Utrecht, Universiteitsweg 100, room: Str. 0.305, 3584CG Utrecht, the Netherlands.
Department of Anatomy, Division Surgical Specialties, University Medical Center Utrecht, Universiteitsweg 100, room: Str. 0.305, 3584CG Utrecht, the Netherlands.
Auton Neurosci. 2019 Nov;221:102580. doi: 10.1016/j.autneu.2019.102580. Epub 2019 Aug 16.
In cardiac ischemia, acute inflammatory responses further increase the detrimental effect on myocardial tissue. Since vagus nerve stimulation (VS) attenuates inflammatory responsiveness this study examines the effect of VS on myocardial damage development in a cardiac ischemia-reperfusion (IR) mouse model.
54 male C57Bl/6j mice were subjected to an IR procedure with or without prior VS. The effects on inflammatory responsiveness, infarct size, cardiac function, neutrophils, lymphocytes and vascular endothelial growth factor (VEGF) in the infarcted myocardium were measured at 48 h after intervention. Group results were compared with unpaired Mann-Whitney or Kruskall-Wallis test.
A significant decrease in inflammatory responsiveness was not verified by decreased TNFα levels in blood from VS and IR treated mice. The percentage infarct size over area at risk was smaller in the group with VS + IR compared with IR (22.4 ± 10.2% vs 37.6 ± 9.0%, p = 0.003). The degree of the reduction in cardiac function was not different between the IR groups with or without VS and no group differences were found in amounts of neutrophils, CD3+ lymphocytes and VEGF in the reperfused mouse heart.
The present study does not provide clear evidence of a reducing role for VS on cardiac function loss. This could mean that VS has a less inhibiting effect on myocardial inflammation than may be expected from the literature.
在心肌缺血中，急性炎症反应进一步增加了对心肌组织的有害影响。由于迷走神经刺激（VS）可减弱炎症反应，本研究旨在观察 VS 对心肌缺血再灌注（IR）小鼠模型中心肌损伤发展的影响。
54 只雄性 C57Bl/6j 小鼠进行 IR 处理，其中部分小鼠在处理前进行 VS。在干预后 48 小时，测量炎症反应、梗死面积、心功能、梗死心肌中的中性粒细胞、淋巴细胞和血管内皮生长因子（VEGF）的变化。组间结果比较采用非配对 Mann-Whitney 或 Kruskal-Wallis 检验。
VS 和 IR 处理小鼠血液中的 TNFα 水平并未降低，表明炎症反应并未显著降低。与 IR 组相比，VS+IR 组的梗死面积百分比较小（22.4±10.2% vs 37.6±9.0%，p=0.003）。IR 组中有无 VS 对心功能的降低程度没有差异，并且在再灌注小鼠心脏中的中性粒细胞、CD3+淋巴细胞和 VEGF 数量也没有组间差异。
本研究没有提供 VS 对心功能丧失具有减轻作用的明确证据。这可能意味着 VS 对心肌炎症的抑制作用比文献预期的要小。
