Laboratório de Biotecnologia, Centro de Biociências e Biotecnologia (CBB), Universidade Estadual Do Norte Fluminense Darcy Ribeiro (UENF), Campos Dos Goytacazes, RJ, 28013-602, Brazil; Unidade de Biologia Integrativa, Setor de Genômica e Proteômica, UENF, Campos Dos Goytacazes, RJ, 28013-602, Brazil.
Laboratório de Biologia Celular e Tecidual, CBB, UENF, Campos Dos Goytacazes, RJ, Brazil.
Plant Physiol Biochem. 2019 Oct;143:109-118. doi: 10.1016/j.plaphy.2019.08.029. Epub 2019 Aug 30.
The use of light-emitting diode (LED) lamps has been shown to be a promising approach for improving somatic embryo maturation during somatic embryogenesis. The aim of this work was to study the influence of the light source on somatic embryo differentiation and its relationship with the differential abundance of proteins in the Carica papaya L. 'Golden' embryogenic callus at 14 days of maturation. The white plus medium-blue (WB) LED and fluorescent lamp treatments produced an average of 82.4 and 47.6 cotyledonary somatic embryos per callus, respectively. A shotgun proteomics analysis revealed 28 upaccumulated and 7 downaccumulated proteins. The proteins upaccumulated in the embryogenic callus matured under the WB LED lamp compared with that matured under the fluorescent lamp included indole-3-acetic acid-amido synthetase (GH3) and actin-depolymerizing factor 2 (ADF2), which are involved in the regulation of auxin levels by auxin conjugation and transport. Additionally, proteins related to energy production (aconitate, ADH1, GAPCp, PKp and TPI), cell wall remodeling (PG and GLPs), and intracellular trafficking (NUP50A, IST1, small GTPases and H-PPase) showed significantly higher abundance in the embryogenic callus incubated under the WB LED lamp than in that incubated under the fluorescent lamp. The results showed that the WB LED lamp improved somatic embryo maturation in association with the differential accumulation of proteins in the C. papaya 'Golden' embryogenic callus.
发光二极管（LED）灯的使用已被证明是改善体细胞胚胎发生过程中体细胞胚胎成熟的一种有前途的方法。本工作旨在研究光源对体细胞胚胎分化的影响及其与 Carica papaya L. 'Golden' 胚性愈伤组织在成熟 14 天时差异表达蛋白的关系。白光加中蓝光（WB）LED 和荧光灯处理分别产生了平均每个愈伤组织 82.4 和 47.6 个子叶体细胞胚。shotgun 蛋白质组学分析显示有 28 个上调蛋白和 7 个下调蛋白。在 WB LED 灯下成熟的胚性愈伤组织中上调的蛋白与在荧光灯下成熟的相比，包括吲哚-3-乙酸酰胺合成酶（GH3）和肌动蛋白解聚因子 2（ADF2），它们参与通过生长素缀合和运输调节生长素水平。此外，与能量产生（顺乌头酸、ADH1、GAPCp、PKp 和 TPI）、细胞壁重塑（PG 和 GLPs）和细胞内运输（NUP50A、IST1、小 GTPases 和 H-PPase）相关的蛋白在 WB LED 灯下孵育的胚性愈伤组织中表现出明显更高的丰度比在荧光灯下孵育的。结果表明，WB LED 灯通过在 C. papaya 'Golden' 胚性愈伤组织中差异积累蛋白来改善体细胞胚胎成熟。
