Gouvea Drielly S, Chagas Kristhiano, Cipriano Jamile L D, Lopes José Carlos, Schmildt Edilson R, Otoni Wagner C, Schmildt Omar, Araujo Caroline P DE, Alexandre Rodrigo S
Departamento de Ciências Agrárias e Biológicas, Centro Universitário Norte do Espírito Santo, Universidade Federal do Espírito Santo/UFES, Rodovia BR 101 Norte, Km 60, Litorâneo, 29932-540 São Mateus, ES, Brazil.
Departamento de Biologia Vegetal, Centro de Ciências Biológicas e da Saúde, Universidade Federal de Viçosa, Campus Universitário, Av. Peter Henry Rolfs, s/n, 36570-900 Viçosa, MG, Brazil.
An Acad Bras Cienc. 2019 Aug 19;91(3):e20180504. doi: 10.1590/0001-3765201920180504.
Somatic embryogenesis from explants from hermaphrodite papaya mother plants is an alternative for the production of true-to-type plants without the need for sexing. This study aimed to analyze hormonal and osmotic inducers in different somatic embryogenesis stages in the commercial hermaphrodite hybrid papaya UENF/Caliman 01. Leaf disks from in vitro shoots originated from ex vitro hermaphrodite plants were cultured in induction medium supplemented with different concentrations of 2,4-D (6, 9, 12, 15, and 18 μM) and 4-CPA (19, 22, 25, 28, and 31 μM). After 90 days, the formation of somatic embryos was verified. The 2,4-D induced the formation of light brown calli with low frequency (20%) of somatic embryogenesis. However, 4-CPA (25 μM) induced 96% of embryogenic calli, which were transferred to maturation medium (MM) and cultured for 30 days. The MM contained ABA (0.5 μM) and AC (15 g L-1) and produced 36.6 somatic embryos callus-1, mainly on cotyledonary stage. Cotyledonary embryos were transferred to germination medium supplemented with gibberellic acid (GA3) (0.0, 1.44, 2.88, and 4.32 µM), and the conversion into plantlets was enhanced with GA3 at 2.88 µM.
从雌雄同株番木瓜母本植株的外植体进行体细胞胚胎发生是一种无需进行性别鉴定即可生产纯种植物的替代方法。本研究旨在分析商业雌雄同株杂交番木瓜UENF/Caliman 01在不同体细胞胚胎发生阶段的激素和渗透诱导剂。将来自离体雌雄同株植物的离体芽上的叶盘培养在添加了不同浓度2,4-D（6、9、12、15和18 μM）和4-CPA（19、22、25、28和31 μM）的诱导培养基中。90天后，验证了体细胞胚的形成。2,4-D诱导形成浅褐色愈伤组织，体细胞胚胎发生频率较低（20%）。然而，4-CPA（25 μM）诱导产生了96%的胚性愈伤组织，将其转移到成熟培养基（MM）中培养30天。MM含有ABA（0.5 μM）和AC（15 g L-1），每个愈伤组织产生36.6个体细胞胚，主要处于子叶期。将子叶胚转移到添加了赤霉素（GA3）（0.0、1.44、2.88和4.32 µM）的萌发培养基中，2.88 µM的GA3提高了其转化为植株的效率。
