Markmann-Mulisch U, Subramanian A R
Max-Planck-Institut für Molekulare Genetik, Abteilung Wittmann, Berlin, (Dahlem), West Germany.
Biochem Int. 1988 Oct;17(4):655-64.
Nucleotide sequence of a 721 base pair segment of maize chloroplast DNA, encoding the putative chloroplast ribosomal protein S11 at physical map position 33.1-33.5 Kbp, is described. A Shine-Dalgarno sequence and computer-derived stem-loop structures of dyad symmetry are present in the spacer region between rpS11 and its 5' upstream gene rpL36. The deduced amino acid sequence of maize chloroplast S11 shows 69%, 66%, 62%, 57%, 48% and 45% sequence identity to the corresponding sequences of tobacco, spinach, pea, liverwort, Escherichia coli and Bacillus subtilis, respectively, and 41% sequence identity to three eukaryotic cytoplasmic ribosomal proteins, S14 of Chinese hamster and of human and rp59 of yeast. Maize chloroplast r-protein S11 is larger than the other published S11s of plants and bacteria, due to the apparent tandem introduction of a short sequence stretch of internal homology.
本文描述了玉米叶绿体DNA中一段721个碱基对片段的核苷酸序列，该片段在物理图谱位置33.1 - 33.5千碱基对处编码假定的叶绿体核糖体蛋白S11。在rpS11及其5'上游基因rpL36之间的间隔区存在一个Shine - Dalgarno序列和计算机推导的二元对称茎环结构。玉米叶绿体S11的推导氨基酸序列与烟草、菠菜、豌豆、地钱、大肠杆菌和枯草芽孢杆菌的相应序列的序列同一性分别为69%、66%、62%、57%、48%和45%，与三种真核细胞质核糖体蛋白，即中国仓鼠和人类的S14以及酵母的rp59的序列同一性为41%。由于内部同源性短序列片段的明显串联引入，玉米叶绿体核糖体蛋白S11比其他已发表的植物和细菌的S11更大。
