Subramanian A R, Steinmetz A, Bogorad L
Nucleic Acids Res. 1983 Aug 11;11(15):5277-86. doi: 10.1093/nar/11.15.5277.
A cloned restriction fragment of maize chloroplast DNA (Bam H1 fragment 5) is shown to contain an open reading frame which encodes a basic protein of 201 amino acid residues with 40-50% sequence homology to E. coli ribosomal protein S4. Based on the experimentally determined sequence homology between the highly conserved bacterial ribosomal protein L12 and its chloroplast homologue (Bartsch M., Kimura, M. and Subramanian, A.R. (1982) Proc. Natl. Acad. Sci. USA 79, 6871), we conclude that this reading frame represents the maize chloroplast S4 gene. The nucleotide sequence of a 1100 base pair DNA segment containing the putative gene is presented.
已表明玉米叶绿体DNA的一个克隆限制片段(Bam H1片段5)含有一个开放阅读框,该开放阅读框编码一种由201个氨基酸残基组成的碱性蛋白,与大肠杆菌核糖体蛋白S4具有40 - 50%的序列同源性。基于实验确定的高度保守的细菌核糖体蛋白L12与其叶绿体同源物之间的序列同源性(Bartsch M.、Kimura M.和Subramanian A.R.(1982年)《美国国家科学院院刊》79,6871),我们得出结论,这个阅读框代表玉米叶绿体S4基因。本文给出了包含该推定基因的一个1100碱基对DNA片段的核苷酸序列。