Departments of Neurobiology, Physiology and Behavior and Physical Medicine and Rehabilitation, University of California, Davis, CA, USA.
Program in Physical Therapy and Departments of Neurology, Biomedical Engineering and Orthopaedic Surgery, Washington University in St. Louis, St. Louis, MO, USA.
Connect Tissue Res. 2020 May-Jul;61(3-4):248-261. doi: 10.1080/03008207.2019.1662409. Epub 2019 Sep 6.
: Skeletal muscle tissue explants have been cultured and studied for nearly 100 years. These cultures, which retain complex tissue structure in an environment suited to precision manipulation and measurement, have led to seminal discoveries of the extrinsic and intrinsic mechanisms regulating contractility, metabolism and regeneration. This review discusses the two primary models of muscle explant: isolated myofiber and intact muscle.: Relevant literature was reviewed and synthesized with a focus on the unique challenges and capabilities of each explant model.: Impactful past, current and future novel applications are discussed.: Experiments using skeletal muscle explants have been integral to our understanding of the fundamentals of muscle physiology. As they are refined and adapted, they are poised to continue to inform the field for years to come.
骨骼肌组织外植体已经被培养和研究了近 100 年。这些培养物在适合精确操作和测量的环境中保留了复杂的组织结构，为调节收缩性、代谢和再生的外在和内在机制的重大发现提供了基础。这篇综述讨论了两种主要的肌肉外植体模型：分离的肌纤维和完整的肌肉。重点讨论了每种外植体模型的独特挑战和能力。讨论了过去、现在和未来有影响力的新型应用。使用骨骼肌外植体的实验对于我们理解肌肉生理学的基本原理至关重要。随着它们的改进和适应，它们有望在未来几年继续为该领域提供信息。
