Seleem Amin Abdou
Zoology Department, Faculty of Science, Sohag University , Sohag , Egypt.
Biology Department, Faculty of Science and Arts, Al-Ula, Taibah University , Medina , Kingdom of Saudi Arabia.
J Histotechnol. 2019 Sep;42(3):104-115. doi: 10.1080/01478885.2019.1619653. Epub 2019 Jul 11.
Methomyl (MET) is a carbamate insecticide which is used as a substitute for organophosphorus compounds to protect crops against insects. The present study aims to evaluate the cytoprotection response of pigment cells and heat shock protein 70 (HSP70) after exposure to MET during the tadpole developmental stages of the Arabian toad, . Three developmental larval stages of the toad were selected and divided into two groups; Control and MET-exposed (MET-EX) tadpoles (10ppm). MET-EX tadpoles showed an increased number of pigment cells in the liver, kidney, anterior eye chamber, and skin tissues as compared to the control. The glycogen content in the developing liver and muscles (myotomes) of MET-EX tadpoles was decreased as compared to the control. In the MET-EX tadpoles, immunohistochemical staining showed an increase of HSP70 expression in the liver hepatocytes, the nucleated red blood cells (nRBC) in kidney glomeruli, the iridocorneal angle of anterior eye chamber, and the skin as compared to the control. The current study concluded that pigment cells and HSP70 represented a cytoprotecting response against MET insecticide during the organ development of tadpoles. Therefore, MET use should be regularly monitored in the environment to protect animals and human from exposure to this insecticide.
灭多威（MET）是一种氨基甲酸酯类杀虫剂，用作有机磷化合物的替代品，以保护作物免受昆虫侵害。本研究旨在评估阿拉伯蟾蜍蝌蚪发育阶段暴露于MET后色素细胞和热休克蛋白70（HSP70）的细胞保护反应。选择蟾蜍的三个发育幼虫阶段并分为两组；对照组和暴露于MET的（MET-EX）蝌蚪（10ppm）。与对照组相比，MET-EX蝌蚪在肝脏、肾脏、眼前房和皮肤组织中的色素细胞数量增加。与对照组相比，MET-EX蝌蚪发育中的肝脏和肌肉（肌节）中的糖原含量降低。在MET-EX蝌蚪中，免疫组织化学染色显示，与对照组相比，肝脏肝细胞、肾小肾小球中的有核红细胞（nRBC）、眼前房的虹膜角膜角和皮肤中HSP70表达增加。当前研究得出结论，在蝌蚪器官发育过程中，色素细胞和HSP70代表了针对MET杀虫剂的细胞保护反应。因此，应定期监测环境中MET的使用情况，以保护动物和人类免受这种杀虫剂的暴露。
