Colorimetric Detection of Organophosphorus Pesticides Based on the Broad-Spectrum Aptamer.

Organophosphorus pesticides (OPs) are an important class of common pesticides. However, due to the abuse of organophosphorus pesticides, pesticide residue problems have been highlighted. In this study, the colorimetric detection method for OPs residues was established based on the broadspectrum aptamer. Through optimization, isocarbophos and omethoate were analyzed to verify the performance of the colorimetric assay. Under optimal conditions, the method proved effective for detecting an isocarbophos range of 50-1000 ng/mL and an omethoate range of 100-500 ng/mL. The protective effects of broad-spectrum aptamers and the split (28-mer P1+26-mer P2) aptamers on gold nanoparticles (AuNPs) were also comparatively studied. The present results suggested that the colorimetric assay based on broad-spectrum aptamer could be applied for a preliminary screening of different kinds of OPs.