Key Laboratory of Biologic Resources Protection and Utilization of Hubei Province, Hubei Minzu University, Enshi, 445000, China.
School of Chemical and Environmental Engineering, Hubei Minzu University, Enshi, 445000, China.
Anal Sci. 2022 Feb;38(2):299-305. doi: 10.2116/analsci.21P204. Epub 2022 Mar 22.
We have developed a new method of one-step simultaneous detection for three pesticides including acetamiprid, atrazine and carbendazim based on organic framework nanomaterial Cu/UiO-66 and three different fluorescent dyes labeled pesticide aptamers. Cu/UiO-66 can be easily combined with pesticide aptamers through strong coordination, and then aptamers were adsorbed to the surface of Cu/UiO-66, which brings the dyes and Cu/UiO-66 into close proximity. Then, the fluorescence of dyes was quenched by Cu/UiO-66. When the target pesticides appeared, the aptamers reacted with corresponding target pesticides and formed special spatial structure, and then the dyes were far away from the surface of Cu/UiO-66 and the fluorescence of dyes is resumed. Thus, the one-step simultaneous detection for three pesticides can be achieved by synchronous fluorescence analysis. The detection limit of acetamiprid, atrazine and carbendazim were 0.1 nmol/L, 1.6 nmol/L, and 0.3 nmol/L, respectively. This method has a good sensitivity, low detection limit, and high selectivity. We have developed a new method of one-step simultaneous detection for three pesticides including acetamiprid, atrazine and carbendazim based on a multi-color fluorescent probe composed of bimetallic organic framework nanomaterials Cu/UiO-66 and three different fluorescent dyes and phosphate double-labeled aptamers of acetamiprid, atrazine and carbendazim.
我们开发了一种基于金属有机骨架纳米材料 Cu/UiO-66 和三种不同荧光染料标记的农药适配体的一步法同时检测三种农药（包括噻虫嗪、莠去津和多菌灵）的新方法。Cu/UiO-66 可以通过强配位与农药适配体轻易结合，然后适配体被吸附到 Cu/UiO-66 的表面，使染料与 Cu/UiO-66 靠近。然后，染料的荧光被 Cu/UiO-66 猝灭。当目标农药出现时，适配体与相应的目标农药发生反应并形成特殊的空间结构，然后染料远离 Cu/UiO-66 的表面，染料的荧光恢复。因此，通过同步荧光分析可以实现三种农药的一步法同时检测。噻虫嗪、莠去津和多菌灵的检测限分别为 0.1 nmol/L、1.6 nmol/L 和 0.3 nmol/L。该方法具有灵敏度高、检测限低、选择性好的特点。我们开发了一种基于双金属有机骨架纳米材料 Cu/UiO-66 和三种不同荧光染料以及噻虫嗪、莠去津和多菌灵的磷酸盐双标记适配体的多色荧光探针，用于一步法同时检测三种农药（包括噻虫嗪、莠去津和多菌灵）。
