在微水混合有机溶剂体系中，通过 CaCl₂/CMC 纳米粒子固定化脂肪酶催化水解制备()-2-苯基丙酸。

Preparation of ()-2-Phenylpropionic Acid by CaCl₂/CMC Nanoparticles Immobilized Lipase-Catalyzed Hydrolysis in Micro Aqueous Mixed Organic Solvent Systems.

机构信息

Hunan Key Laboratory of Biomedical Nanomaterials and Devices, Hunan University of Technology, Zhuzhou 412007, PR China.

College of Life Sciences and Chemistry, Hunan University of Technology, Zhuzhou 412007, PR China.

出版信息

J Nanosci Nanotechnol. 2020 Mar 1;20(3):1899-1906. doi: 10.1166/jnn.2020.17343.

DOI:10.1166/jnn.2020.17343

PMID:31492359

Abstract

lipase was immobilized in this study using CaCl₂/CMC nanoparticles that yielded a lipase loading capacity of 127 mg/g, with better thermal stability and activity of 91.8%. The hydrolysis of racemic 2-phenylpropionic acid isopropyl ester by free and immobilized lipase was investigated in the mixed organic-solvent composed of isooctane and methyl tert-butyl ether (9.5:0.5, V/V). The optimal conditions were 35 °C and pH 7.5 for free lipase hydrolysis. We obtained ()-2-phenylpropionic acid with 44.85% conversion, 95.75% enantiomeric excess and enantiomeric ratio of 112. The CaCl₂/CMC nanoparticles immobilized lipase possesses high enantioselectivity, with = 237 at 40 °C and pH 7.5. It was efficiently reusable in four cycles and appropriately enhanced enantioselectivity within 120-240.

摘要

在本研究中，脂肪酶通过 CaCl₂/CMC 纳米粒子固定化，脂肪酶的载量为 127 mg/g，热稳定性和活性提高到 91.8%。考察了游离脂肪酶和固定化脂肪酶在异辛烷和甲基叔丁基醚（9.5:0.5，V/V）组成的混合有机溶剂中对消旋 2-苯基丙酸异丙酯的水解作用。游离脂肪酶水解的最佳条件为 35°C 和 pH7.5。我们得到（）-2-苯基丙酸，转化率为 44.85%，对映体过量值为 95.75%，对映体比为 112。CaCl₂/CMC 纳米粒子固定化脂肪酶具有高对映选择性，在 40°C 和 pH7.5 时，=237。它在 4 个循环中可有效重复使用，并在 120-240 范围内适当提高了对映选择性。