Brun A, Sandberg S
Laboratory of Clinical Biochemistry, University of Bergen, Norway.
J Photochem Photobiol B. 1988 Jul;2(1):33-41. doi: 10.1016/1011-1344(88)85035-8.
Erythrocytes from patients with erythropoietic protoporphyria (EPP) contain large amounts of protoporphyrin. By fluorescence microscopy it has been found that the erythrocytes show red fluorescence which fades very rapidly. During irradiation of erythrocytes from patients with EPP, a decrease in the fluorescence intensity and a red shift in the fluorescence emission maximum is observed. Since the human eye has a diminishing sensitivity in the red part of the spectrum, the red shift will augment the fluorescence decrease observed in the microscope. The decrease in fluorescence intensity is greater than what would be expected by photobleaching of protoporphyrin alone. We suggest that the rapid fading of fluorocytes observed in a fluorescence microscope can be explained both by photoinduced detachment of protoporphyrin from hemoglobin followed by a redistribution to the erythrocyte membrane and by protoporphyrin photobleaching.
红细胞生成性原卟啉病（EPP）患者的红细胞含有大量原卟啉。通过荧光显微镜观察发现，这些红细胞呈现红色荧光，且褪色非常迅速。在对EPP患者的红细胞进行照射时，可观察到荧光强度降低以及荧光发射最大值出现红移。由于人眼对光谱红色部分的敏感度逐渐降低，这种红移会增强在显微镜下观察到的荧光减弱现象。荧光强度的降低幅度大于仅由原卟啉光漂白所预期的幅度。我们认为，在荧光显微镜下观察到的荧光细胞快速褪色现象，既可以通过原卟啉从血红蛋白上的光诱导脱离，随后重新分布到红细胞膜上，也可以通过原卟啉的光漂白来解释。
