光敏色素的分子拓扑结构：生色团与脱辅基蛋白。

The molecular topography of phytochrome: chromophore and apoprotein.

作者信息

Song P S

机构信息

Department of Chemistry, School of Biological Sciences, University of Nebraska, Lincoln 68588-0304.

出版信息

J Photochem Photobiol B. 1988 Jul;2(1):43-57. doi: 10.1016/1011-1344(88)85036-x.

DOI:10.1016/1011-1344(88)85036-x

PMID:3149301

Abstract

Phytochrome serves as the photochromic receptor for a number of morphogenic and developmental responses to red light in higher plants. The photoreversible phototransformation of 124 kDa oat phytochrome involves several structural changes in the chromophore and the apoprotein, including a configurational/conformational isomerization and secondary/tertiary structural changes respectively. For example, there appears to be a specific interaction between the chromophore and the amino terminus segment in the Pfr form of phytochrome, which results in a photoreversible peptide folding of the amino terminus peptide chain. Other structural changes also accompany the phototransformation, as has been probed by peptide mapping, phosphorylation, and monoclonal antibodies.

摘要

光敏色素作为高等植物中对红光的多种形态发生和发育反应的光致变色受体。124 kDa燕麦光敏色素的光可逆光转化涉及发色团和脱辅基蛋白的几种结构变化，分别包括构型/构象异构化和二级/三级结构变化。例如，在光敏色素的Pfr形式中，发色团与氨基末端片段之间似乎存在特定相互作用，这导致氨基末端肽链发生光可逆的肽折叠。如通过肽图谱分析、磷酸化和单克隆抗体所探测到的，其他结构变化也伴随着光转化。