希腊出现同时产 NDM-1 和 OXA-48 碳青霉烯酶的肺炎克雷伯菌 ST11 型。
Emergence of Klebsiella pneumoniae ST11 co-producing NDM-1 and OXA-48 carbapenemases in Greece.
机构信息
Department of Microbiology, AHEPA University Hospital, S. Kiriakidi Str. 1, 54636 Thessaloniki, Greece.
Department of Microbiology, AHEPA University Hospital, S. Kiriakidi Str. 1, 54636 Thessaloniki, Greece.
出版信息
J Glob Antimicrob Resist. 2019 Dec;19:81-82. doi: 10.1016/j.jgar.2019.08.020. Epub 2019 Sep 4.
OBJECTIVES
Klebsiella pneumoniae is a well-known pathogen frequently implicated in serious life-threatening nosocomial infections. Here we present a K. pneumoniae isolate (AHEPA1046) co-harbouring bla and bla isolated from a blood sample of an inpatient in Thessaloniki, Greece.
METHODS
Whole-genome sequencing (WGS) was performed using an Illumina MiniSeq Sequencing System. Multilocus sequence typing (MLST) was performed using a BLAST-based approach, and antimicrobial resistance genes and plasmid replicons were identified by ResFinder and PlasmidFinder, respectively. The Rapid Annotation using Subsystem Technology (RAST) v.2.0 server was used for genome annotation.
RESULTS
WGS analysis revealed the complete resistome of K. pneumoniae AHEPA1046. The strain harboured bla and bla together with 16 additional antimicrobial resistance genes and was resistant to carbapenems, aminoglycosides, quinolones, macrolides, tetracyclines, trimethoprim, fosfomycin and phenicols. Moreover, it was classified as ST11.
CONCLUSION
This is the first report of a K. pneumoniae clinical isolate from Greece co-producing NDM-1 and OXA-48 carbapenemases and is one of a few reported worldwide.
目的
肺炎克雷伯菌是一种众所周知的病原体，常与严重的危及生命的医院获得性感染有关。在这里，我们从希腊塞萨洛尼基的一名住院患者的血液样本中分离出一株同时携带 bla 和 bla 的肺炎克雷伯菌（AHEPA1046）。
方法
使用 Illumina MiniSeq 测序系统进行全基因组测序（WGS）。采用基于 BLAST 的方法进行多位点序列分型（MLST），并分别使用 ResFinder 和 PlasmidFinder 鉴定抗生素耐药基因和质粒复制子。使用 Rapid Annotation using Subsystem Technology（RAST）v.2.0 服务器进行基因组注释。
结果
WGS 分析揭示了肺炎克雷伯菌 AHEPA1046 的完整耐药组。该菌株携带 bla 和 bla 以及另外 16 种抗生素耐药基因，对碳青霉烯类、氨基糖苷类、喹诺酮类、大环内酯类、四环素类、甲氧苄啶、磷霉素和酚类抗生素均具有耐药性。此外，它被归类为 ST11。
结论
这是首例来自希腊的同时产生 NDM-1 和 OXA-48 碳青霉烯酶的肺炎克雷伯菌临床分离株的报告，也是世界上少数几例报告之一。
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