Tsilipounidaki Katerina, Florou Zoi, Skoulakis Anargyros, Fthenakis George C, Miriagou Vivi, Petinaki Efthymia
Faculty of Medicine, University of Thessaly, 41500 Larissa, Greece.
Veterinary Faculty, University of Thessaly, 43100 Karditsa, Greece.
Microorganisms. 2023 Feb 17;11(2):516. doi: 10.3390/microorganisms11020516.
The objective of the present study was to genetically characterize ten NDM-1 producing isolates, recovered from patients in a hospital in Central Greece during the period 2017 to 2021.The isolates were studied by whole genome sequencing to obtain multi-locus sequencing typing (MLST), identification of -environment, resistome and plasmid content. MLST analysis showed the presence of eight sequence types: ST46* (two isolates), ST46, ST744, ST998, ST410, ST224, ST4380, ST683 and ST12 (one isolate each). Apart of the presence of , the isolates carried a combination of various to -lactams encoding resistance genes: , bla, , , , , and . Additionally, plurality of resistance genes to aminoglycosides, macrolides, rifamycin, phenicols, sulfonamides and tetracycline was detected. The presence of multiple replicons was observed, with predominance of IncFII and IncFIB. Analysis of genetic environment of the isolates showed that seven had 100% identity with the pS-3002cz plasmid (Accession Number KJ 958927), two with the pB-3002cz plasmid (Accession Number KJ958926) and one with the pEc19397-131 plasmid (Accession Number MG878866). Τhis latter plasmid was derived by the fusion of two, previously identified, plasmids, pAMPD2 and pLK75 (Accession Numbers CP078058 and KJ440076, respectively). The diversity of clones and plasmids of NDM-1 producing isolated from patients in Greece indicates a continuous horizontal gene transfer.
本研究的目的是对2017年至2021年期间从希腊中部一家医院的患者中分离出的10株产NDM-1菌株进行基因特征分析。通过全基因组测序对这些菌株进行研究，以获得多位点测序分型（MLST）、鉴定其环境、耐药基因组和质粒含量。MLST分析显示存在8种序列类型：ST46*（2株）、ST46、ST744、ST998、ST410、ST224、ST4380、ST683和ST12（各1株）。除了存在 外，这些菌株携带多种β-内酰胺类耐药基因的组合：bla 、bla 、bla 、bla 、bla 、bla 、bla 和bla 。此外，还检测到多个对氨基糖苷类、大环内酯类、利福霉素、酚类、磺胺类和四环素的耐药基因。观察到存在多个复制子，以IncFII和IncFIB为主。对这些菌株的遗传环境分析表明，7株与pS-3002cz质粒（登录号KJ 958927）有100%的同一性，2株与pB-3002cz质粒（登录号KJ958926）有100%的同一性，1株与pEc19397-131质粒（登录号MG878866）有100%的同一性。后一种质粒是由先前鉴定的两种质粒pAMPD2和pLK75（登录号分别为CP078058和KJ440076）融合而成。从希腊患者中分离出的产NDM-1菌株的克隆和质粒多样性表明存在持续的水平基因转移。
