Graduate Program in Toxicological Biochemistry, Universidade Federal de Santa Maria (UFSM), Santa Maria, RS 97105-900, Brazil.
Graduate Program in Physiology and Pharmacology, Universidade Federal de Santa Maria - UFSM, Santa Maria, RS 97105-900, Brazil.
Comp Biochem Physiol C Toxicol Pharmacol. 2020 Jan;227:108614. doi: 10.1016/j.cbpc.2019.108614. Epub 2019 Sep 4.
Aflatoxin BAFB) is one of the most important mycotoxins due to its hepatotoxic and carcinogenic effects on animals. The effect of dietary supplementation with vegetable choline (VC) at 400, 800, and 1200 mg/kg against the deleterious effects of AFB (2 ppm/kg diet) in the liver of Nile tilapia (Oreochromis niloticus) was studied. The experimental period was 81 days, and the diet with VC was offered to the fish for 60 days prior to challenge with AFB. Diets with AFB were tested in three replications and animals were analyzed at days 14 and 21 of dietary intake. The addition of VC to tilapia diet increased body weight (days 30 and 60 pre-challenge and day 21 post-challenge). The group fed aflatoxin-contaminated diet presented significantly reduced antioxidant enzymes and increased reactive oxygen species (ROS) levels, thiobarbituric acid reactive species (TBARS) levels, and protein carbonyl (PC) content in the liver. Dietary supplementation with VC at 800 and 1200 mg/kg demonstrated a significant protective effect, avoiding the increase of ROS, TBARS, and PC levels in the liver of tilapia from the aflatoxin contaminated groups. Thus, dietary VC supplementation may be used in tilapia to increase antioxidant status and reduce the negative effects caused by AFB toxicity. Based on the findings, it is recommended to use VC as a food supplement for Nile tilapia in order to avoid AFB toxication. In addition, decreased aflatoxin toxicity can be attributed to the VC antioxidant property.
黄曲霉毒素 BAFB）是最重要的霉菌毒素之一，因为它对动物具有肝毒性和致癌作用。研究了膳食补充蔬菜胆碱（VC）在 400、800 和 1200mg/kg 对尼罗罗非鱼（Oreochromis niloticus）肝脏中 AFB（2ppm/kg 饮食）的有害影响。实验期为 81 天，在与 AFB 挑战前，VC 饮食提供给鱼类 60 天。用 AFB 测试了三种重复的饮食，动物在饮食摄入的第 14 天和第 21 天进行分析。在罗非鱼饮食中添加 VC 增加了体重（挑战前第 30 天和第 60 天以及挑战后第 21 天）。喂食黄曲霉毒素污染饮食的组表现出抗氧化酶显著降低，并且肝脏中的活性氧（ROS）水平、硫代巴比妥酸反应性物质（TBARS）水平和蛋白质羰基（PC）含量增加。在 800 和 1200mg/kg 时，膳食补充 VC 表现出显著的保护作用，避免了 ROS、TBARS 和 PC 水平在来自黄曲霉毒素污染组的罗非鱼肝脏中的增加。因此，在罗非鱼中补充 VC 可能会增加抗氧化状态并减少 AFB 毒性引起的负面影响。基于这些发现，建议将 VC 用作尼罗罗非鱼的食品补充剂，以避免 AFB 中毒。此外，黄曲霉毒素毒性的降低可归因于 VC 的抗氧化特性。
