Dazuk Vanessa, Tarasconi Lara, Molosse Vitor Luiz, Cécere Bruno Giorgio Oliveira, Deolindo Guilherme Luiz, Strapazzon João Vitor, Bottari Nathieli Bianchi, Bissacotti Bianca Fagan, Schetinger Maria Rosa Chitolina, Sareta Laércio, Mendes Ricardo Evandro, Vedovatto Marcelo, Gloria Eduardo Micotti, Paiano Diovani, Galli Gabriela Miotto, Da Silva Aleksandro Schafer
Programa de Pós-graduação em Zootecnia, Universidade do Estado de Santa Catarina (UDESC), Chapecó 89815-630, Brazil.
Departamento de Zootecnia, Universidade do Estado de Santa Catarina (UDESC), Chapecó 89815-630, Brazil.
Animals (Basel). 2023 Sep 25;13(19):3010. doi: 10.3390/ani13193010.
(1) Background: This study's objective was to determine whether adding vegetable biocholine (VB) to pigs' diets would minimize the negative effects caused by daily aflatoxin (B1 + B2) intake. (2) Methods: We used seventy-two whole male pigs weaned at an average of 26 days and divided them into four groups with six replicates each (2 × 2 factorial). The treatments were identified as Afla0VB0 (negative control, without aflatoxin and without VB); Afla500VB0 (positive control, 500 µg/kg of aflatoxins; Afla0VB800 (800 mg/kg of VB); and Afla500VB800 (500 µg/kg of aflatoxin +800 mg/kg of VB). (3) Results: In the first 20 days of the experiment, only the pigs from Afla500VB0 had less weight gain and less feed consumption, different from the 30th to 40th day, when all treatments had lower performance than the negative control. In the liver, higher levels of oxygen-reactive species and lipid peroxidation were observed in Afla500VB0, associated with greater activity of the enzymes alanine aminotransferase and aspartate aminotransferase. In the jejunum, oxidative stress was associated with nitrous stress in Afla500VB0. An increase in splenic glutathione S-transferase activity in the Afla500VB800 animals was observed. (4) Conclusions: Consuming a diet contaminated with 500 µg/kg of aflatoxin influences the health and performance in the nursing phase in a silent way; however, it generates high economic losses for producers. When VB was added to the pigs' diet in the face of an aflatoxin challenge, it showed hepatoprotective potential.
(1) 背景：本研究的目的是确定在猪的日粮中添加植物生物胆碱（VB）是否能将每日黄曲霉毒素（B1 + B2）摄入量造成的负面影响降至最低。(2) 方法：我们使用了72头平均26日龄断奶的雄性仔猪，将它们分为四组，每组六个重复（2×2析因设计）。处理组分别为Afla0VB0（阴性对照，无黄曲霉毒素和无VB）；Afla500VB0（阳性对照，500 µg/kg黄曲霉毒素）；Afla0VB800（800 mg/kg VB）；以及Afla500VB800（500 µg/kg黄曲霉毒素 + 800 mg/kg VB）。(3) 结果：在实验的前20天，只有Afla500VB0组的猪体重增加较少且采食量较低，与第30至40天不同，此时所有处理组的性能均低于阴性对照。在肝脏中，Afla500VB0组观察到较高水平的氧自由基和脂质过氧化，这与丙氨酸转氨酶和天冬氨酸转氨酶的活性增加有关。在空肠中，Afla500VB0组的氧化应激与亚硝酸盐应激有关。观察到Afla500VB800组动物脾脏谷胱甘肽S-转移酶活性增加。(4) 结论：摄入含有500 µg/kg黄曲霉毒素的日粮会在哺乳阶段以隐性方式影响健康和性能；然而，这会给生产者带来巨大的经济损失。当面对黄曲霉毒素挑战时，在猪的日粮中添加VB显示出肝脏保护潜力。
