School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China; Department of Environmental Engineering, Zhejiang University, Hangzhou 310058, China.
School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, China.
Bioresour Technol. 2019 Dec;293:122096. doi: 10.1016/j.biortech.2019.122096. Epub 2019 Aug 31.
The microbial community characteristics, functional and antibiotic resistance genes (ARGs), anammox performance under individual and combined oxytetracycline (OTC) and sulfamethoxazole (SMX) were tested under environmentally relevant levels. The results showed that anammox performance was inhibited when the OTC or SMX concentration increased from 0.5 to 1.0 mg L. The absolute abundance of tetX in OTC (3.03 × 10 copies mg), SMX (2.80 × 10 copies mg) and OTC + SMX (2.03 × 10 copies mg) was the highest and one more order of magnitude higher than that of tetG, tetM, intI1, or sul2. The anammox performance in the presence of OTC or SMX was lower than that sum of their independent effects. The enrichment of sludge resistomes with prolonged exposure time and increasing OTC and SMX doses might be due to succession of bacterial hosts and potential elevation of ARGs by horizontal transfer.
在环境相关浓度下，测试了单个和组合土霉素（OTC）和磺胺甲恶唑（SMX）条件下微生物群落特征、功能和抗生素抗性基因（ARGs）、厌氧氨氧化性能。结果表明，当 OTC 或 SMX 浓度从 0.5 增加到 1.0mg/L 时，厌氧氨氧化性能受到抑制。在 OTC（3.03×10 拷贝 mg）、SMX（2.80×10 拷贝 mg）和 OTC+SMX（2.03×10 拷贝 mg）中 tetX 的绝对丰度最高，比 tetG、tetM、intI1 或 sul2 高一个数量级。在存在 OTC 或 SMX 的情况下，厌氧氨氧化性能低于其各自独立作用的总和。由于细菌宿主的连续富集和水平转移可能导致潜在的 ARGs 升高，因此长时间暴露于 OTC 和 SMX 剂量增加会导致污泥抗性组的富集。
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