Drake Keri A, Fessler Alicia R, Carroll Thomas J
Division of Pediatric Nephrology, University of Texas Southwestern Medical Center, Dallas, TX, United States.
Department of Internal Medicine (Nephrology), University of Texas Southwestern Medical Center, Dallas, TX, United States; Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, United States.
Methods Cell Biol. 2019;154:121-143. doi: 10.1016/bs.mcb.2019.06.002. Epub 2019 Jul 15.
Lineage tracing has resulted in fundamental discoveries in kidney development and disease and remains a powerful technique to study mechanisms of organogenesis, homeostasis, and repair/regeneration. Following decades of research on the cellular and molecular regulation of renal organogenesis, the kidney has become one of the most well-characterized organs, resulting in exciting advancements in pluripotent stem cell differentiation, tissue bioengineering, and the potential for developing novel regenerative therapies for kidney disease. Lineage tracing, or the labeling of progeny cells arising from a single cell or group of cells, allows for spatial and temporal analyses of dynamic in vivo and in vitro processes. As lineage tracing techniques expand across disciplines of developmental biology, stem cell biology, and regenerative medicine, careful experimental design and interpretation, along with an understanding of the basic principles and technical limitations, are essential for utilizing genetically complex lineage tracing models to further understand kidney development and disease.
谱系追踪已在肾脏发育和疾病研究中带来了根本性的发现，并且仍然是研究器官发生、内稳态以及修复/再生机制的强大技术。经过数十年对肾脏器官发生的细胞和分子调控的研究，肾脏已成为特征最为明确的器官之一，这在多能干细胞分化、组织生物工程以及开发针对肾脏疾病的新型再生疗法的潜力方面带来了令人兴奋的进展。谱系追踪，即对源自单个细胞或一组细胞的子代细胞进行标记，能够对体内和体外的动态过程进行空间和时间分析。随着谱系追踪技术在发育生物学、干细胞生物学和再生医学等学科中的扩展，精心的实验设计和解读，以及对基本原理和技术局限性的理解，对于利用遗传复杂的谱系追踪模型来进一步了解肾脏发育和疾病至关重要。
