Department of Pediatrics, The Affiliated Hospital of Qingdao University of Shandong Province, Qingdao, China.
Department of Obstetrics and Gynecology, Binzhou Medical University Hospital of Shandong Province, Binzhou, China.
Microb Pathog. 2019 Nov;136:103709. doi: 10.1016/j.micpath.2019.103709. Epub 2019 Sep 5.
The impact of the gut microbiota on recurrent respiratory tract infection (RRTI) remains to be fully elucidated.
To characterize the gut microbiota in patients with RRTI, fecal samples from 26 patients with RRTI and 23 healthy volunteers were profiled using the Illumina MiSeq platform. Beta diversity (Principal Component Analysis (PCA), Principal Co-ordinates Analysis (PCoA), Non-metric multidimensional scaling (NMDS)) analysis showed that the bacterial community structure segregated differently between the RRTI and control groups.
Results from alpha diversity analysis revealed lower microbiota diversity in samples from RRTI patients than in normal controls. Taxonomic analysis illustrated that the abundance of six phyla (Firmicutes, Proteobacteria, Bacteroidetes, Actinobacteria, Verrucomicrobia, Tenericutes) and four genera (Enterococcus, Faecalibacterium, Bifidobacterium, Eubacterium were significantly different between these two groups. In addition, Enterococcus (P < 0.001) was more enriched in the RRTI group, whereas the abundances of Eubacterium (P < 0.001), Faecalibacterium (0.01 < P < 0.05) and Bifidobacterium (0.01 < P < 0.05) were reduced in the RRTI group compared to those in the normal control group. The performance of the model was assessed using ROC analysis, and Enterococcus, Eubacterium and Bifidobacterium achieved AUC values of 0.860, 0.820, and 0.689, respectively.
These results provide fundamental evidence in support of intestinal microbiota dysbiosis in children with RRTI.
肠道微生物群对反复呼吸道感染（RRTI）的影响仍有待充分阐明。
为了描述 RRTI 患者的肠道微生物群，我们使用 Illumina MiSeq 平台对 26 例 RRTI 患者和 23 例健康志愿者的粪便样本进行了分析。β多样性（主成分分析（PCA）、主坐标分析（PCoA）、非度量多维尺度分析（NMDS））分析表明，RRTI 组和对照组的细菌群落结构明显不同。
α多样性分析结果显示，RRTI 患者样本中的微生物多样性低于正常对照组。分类分析表明，两组之间六个门（厚壁菌门、变形菌门、拟杆菌门、放线菌门、疣微菌门、柔膜菌门）和四个属（肠球菌属、粪杆菌属、双歧杆菌属、真杆菌属）的丰度存在显著差异。此外，肠球菌属（P<0.001）在 RRTI 组中更为丰富，而真杆菌属（P<0.001）、粪杆菌属（0.01<P<0.05）和双歧杆菌属（0.01<P<0.05）的丰度在 RRTI 组中低于正常对照组。使用 ROC 分析评估模型的性能，肠球菌属、真杆菌属和双歧杆菌属的 AUC 值分别为 0.860、0.820 和 0.689。
这些结果为 RRTI 患儿肠道微生物群失调提供了基础证据。
