Department of Hepatobiliary surgery, The First Affiliated Hospital of Chonqing Medical University, Chongqing, China.
Department of General surgery, West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu, China.
Postgrad Med J. 2020 Feb;96(1132):79-83. doi: 10.1136/postgradmedj-2019-136905. Epub 2019 Sep 7.
Long non-coding RNAs (LncRNAs) have been recently implicated as having oncogenic and tumour suppressor roles. LncRNA LOC285194 (LOC285194) expression was significantly reduced in a variety of tumour tissues and cell lines, which promotes cell proliferation and migration. The aim of the present study is to examine the expression pattern of LOC285194 and its clinical significance in hepatocellular carcinoma (HCC) patients after curative liver resection.
We examined the expression of LOC285194 in 120 HCC samples and controls from adjacent non-tumour tissues using real-time quantitative reverse transcription-PCR and analysed its correlation with clinical parameters and prognosis in these patients who have undergone curative hepatic resection with a median follow-up of 3.5 years.
The expression level of LOC285194 was significantly lower in tumour tissues and four liver cancer cell lines compared with adjacent normal tissues and normal liver cell line. Furthermore, a low expression of LOC285194 was significantly correlated with advanced tumour stage, microvascular invasion, tumour number and differentiation. Additionally, survival analysis showed that patients with low LOC285194 expression had a significantly worse overall and disease-free survival. Moreover, univariate and multivariate analyses showed that decreased expression of LOC285194 was an independent predictor of long-term survival.
The low expression level of LOC285194 might be a novel candidate biomarker for predicting tumour progression and poor prognosis in HCC patients who have undergone hepatectomy and might be a potential target for gene therapy.
长链非编码 RNA（LncRNA）最近被认为具有致癌和肿瘤抑制作用。在各种肿瘤组织和细胞系中，LncRNA LOC285194（LOC285194）的表达显著降低，促进细胞增殖和迁移。本研究旨在检测 LOC285194 在肝癌（HCC）患者根治性肝切除术后的表达模式及其临床意义。
我们使用实时定量逆转录 PCR 检测了 120 例 HCC 样本和来自相邻非肿瘤组织的对照样本中 LOC285194 的表达，并分析了其与这些患者临床参数和预后的相关性，这些患者在中位随访 3.5 年后接受了根治性肝切除。
与相邻正常组织和正常肝细胞系相比，肿瘤组织和四种肝癌细胞系中 LOC285194 的表达水平显著降低。此外，LOC285194 表达水平低与肿瘤晚期、微血管侵犯、肿瘤数量和分化程度显著相关。此外，生存分析显示，LOC285194 低表达的患者总生存和无病生存明显较差。此外，单因素和多因素分析表明，LOC285194 表达降低是长期生存的独立预测因子。
LOC285194 的低表达水平可能是预测 HCC 患者肝切除术后肿瘤进展和预后不良的新候选生物标志物，可能是基因治疗的潜在靶点。
