长链非编码 RNA LOC285194 促进卵巢上皮性癌细胞进展及其凋亡信号通路。
Long Non-coding RNA LOC285194 Promotes Epithelial Ovarian Cancer Progression the Apoptosis Signaling Pathway.
机构信息
Department of Obstetrics and Gynecology, Dongguk University College of Medicine, Goyang, Republic of Korea.
Department of Obstetrics and Gynecology, Bucheon St. Mary Hospital, Catholic University Medical College, Bucheon, Republic of Korea.
出版信息
In Vivo. 2022 Jan-Feb;36(1):121-131. doi: 10.21873/invivo.12683.
BACKGROUND/AIM: To explore the molecular mechanism and clinical significance of a newly identified lncRNA LOC285194 in epithelial ovarian cancer (EOC).
MATERIALS AND METHODS
LOC285194 transcript levels were analyzed in EOC cells compared to normal cells. Small interfering RNAs were used to suppress LOC285194 expression. Levels of apoptosis-related proteins were determined by western blot. LOC285194 expression in ovarian cancer and non-tumor tissues were compared with clinicopathologic and survival data.
RESULTS
Knockdown of LOC285194 decreased cell migration and proliferation, enhanced reactive oxygen species production and resulted in increased levels of proteins of the extrinsic apoptotic signaling pathway. LOC285194 expression level was higher in ovarian cancer tissues compared to control. Overall survival was significantly shorter in patients with high LOC285194 expression. Lymph node metastasis and high LOC285194 expression were significant prognostic factors of mortality (HR=4.614 and 5.880; p=0.026 and p=0.002, respectively).
CONCLUSION
LOC285194 can promote the progression of EOC via an anti-apoptotic mechanism. It may serve as a novel biomarker for predicting prognosis of EOC.
背景/目的：探索新鉴定的长链非编码 RNA LOC285194 在卵巢上皮性癌（EOC）中的分子机制和临床意义。
材料和方法
比较 EOC 细胞与正常细胞中 LOC285194 的转录水平。使用小干扰 RNA 抑制 LOC285194 的表达。通过 Western blot 测定凋亡相关蛋白的水平。比较卵巢癌和非肿瘤组织中 LOC285194 的表达与临床病理和生存数据。
结果
敲低 LOC285194 可降低细胞迁移和增殖，增强活性氧的产生，并导致外源性凋亡信号通路蛋白水平升高。与对照相比，卵巢癌组织中 LOC285194 的表达水平更高。LOC285194 高表达的患者总生存期明显缩短。淋巴结转移和 LOC285194 高表达是死亡率的显著预后因素（HR=4.614 和 5.880；p=0.026 和 p=0.002）。
结论
LOC285194 可通过抗凋亡机制促进 EOC 的进展。它可能成为预测 EOC 预后的新型生物标志物。
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