Rapak A, Szewczuk A, Kolarz B N, Trochimczuk A, Wojaczyńska M
Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw.
Arch Immunol Ther Exp (Warsz). 1987;35(6):811-8.
Among several various acrylic copolymer used as carriers for anti-bovine serum albumin the best are copolymers obtained from acrylonitrile and ethyl or butyl acrylate crosslinked with divinylbenzene which after aminolysis with di- or triamines were activated with glutardialdehyde. The immunoadsorbents thus obtained were successfully used for purification of bovine serum albumin from bovine serum or from crude fraction of the albumin by immunoaffinity chromatography. Electrophoretically pure bovine albumin was eluted from immunoadsorbent column with deionised water and with glycine/HCl buffer, pH 2.5. The albumin was contamined with less than 0.1% of bovine immunoglobulins and with 0.05% of rabbit immunoglobulins. No contamination with rabbit immunoglobulin was observed when immunoadsorbents were pre-treated with sodium borohydride.
在用作抗牛血清白蛋白载体的几种不同丙烯酸共聚物中，最好的是由丙烯腈与丙烯酸乙酯或丁酯与二乙烯基苯交联得到的共聚物，这些共聚物在用二胺或三胺进行氨解后用戊二醛活化。由此获得的免疫吸附剂已成功用于通过免疫亲和色谱从牛血清或白蛋白粗级分中纯化牛血清白蛋白。用去离子水和pH 2.5的甘氨酸/盐酸缓冲液从免疫吸附柱上洗脱电泳纯的牛白蛋白。该白蛋白被污染的牛免疫球蛋白少于0.1%，兔免疫球蛋白为0.05%。当用硼氢化钠对免疫吸附剂进行预处理时，未观察到兔免疫球蛋白的污染。
