小鼠中θ抗原基因的定位。III. Thy-1相对于Lap-1和Mpi-1的位置。

Location of the gene for theta antigen in the mouse. III. The position of Thy-1 relative to Lap-1 and Mpi-1.

作者信息

Douglas T C, Dawson P E

出版信息

J Hered. 1979 Jul-Aug;70(4):250-4. doi: 10.1093/oxfordjournals.jhered.a109248.

DOI:10.1093/oxfordjournals.jhered.a109248

Abstract

The position of the Thy-1 (theta cell surface antigen) locus on chromosome 9 of the mouse was determined relative to the biochemical markers Lap-1 (leucine arylaminopeptidase) and Mpi-1 (mannosephosphate isomerase). Four-point backcrosses using both male and female heterozygotes showed that the order of these loci is Lap-1--Thy-1--Mpi-1. By observing the segregation of alleles at the Mod-1 (cytoplasmic malic enzyme) locus, which is known to lie distal to these three markers, it was possible to show that Lap-1 is at the centromeric end of this gene group. The overall map for this portion of chromosome 9 as determined by these crosses is: Lap-1--5--Thy-1--7--Mpi-1--14--Mod-1.

摘要

确定了小鼠9号染色体上Thy-1（θ细胞表面抗原）基因座相对于生化标记Lap-1（亮氨酸芳基氨基肽酶）和Mpi-1（甘露糖磷酸异构酶）的位置。使用雄性和雌性杂合子进行的四点回交表明，这些基因座的顺序是Lap-1--Thy-1--Mpi-1。通过观察已知位于这三个标记远端的Mod-1（细胞质苹果酸酶）基因座上等位基因的分离情况，发现Lap-1位于该基因组的着丝粒端。通过这些杂交确定的9号染色体这一部分的总图为：Lap-1--5--Thy-1--7--Mpi-1--14--Mod-1。