Bojanovski M, Gregg R E, Wilson D M, Brewer H B
Medizinische Hochschule Hannover, Department of Biochemistry, FRG.
Clin Chim Acta. 1988 Dec 15;178(2):159-69. doi: 10.1016/0009-8981(88)90222-7.
A semi-automated competitive enzyme-linked immunosorbent assay for human plasma apolipoprotein (Apo) A-I has been developed which utilizes nondelipidated samples, microtiter plates, commercially available monoclonal antibodies and alkaline phosphatase conjugated second antibody. The working range of the assay is 5-100 ng of Apo A-I. The range of plasma concentrations for plasma Apo A-I was 1.21 +/- 0.34 g/l for a random sample of 40 healthy adults. Intra- and inter-assay coefficients of variation (CV) were 4 and 7%, respectively. There was a good correlation between this assay and a radial immunodiffusion assay (r = 0.96). The assay is suitable for measurement of apolipoprotein A-I in either normal or pathological plasma, lipoprotein density classes, and for cell biological and molecular biological investigations.
已开发出一种用于人血浆载脂蛋白（Apo）A-I的半自动竞争性酶联免疫吸附测定法，该方法使用未脱脂样品、微量滴定板、市售单克隆抗体和碱性磷酸酶偶联的二抗。该测定法的工作范围是5-100 ng的Apo A-I。对40名健康成年人的随机样本进行检测，血浆Apo A-I的血浆浓度范围为1.21±0.34 g/l。批内和批间变异系数（CV）分别为4%和7%。该测定法与放射免疫扩散测定法之间具有良好的相关性（r = 0.96）。该测定法适用于正常或病理血浆、脂蛋白密度类别中载脂蛋白A-I的测量，也适用于细胞生物学和分子生物学研究。
