Department of Pharmaceutical Technology, Centre for Excellence in Nanobio Translational Research (CENTRE), University College of Engineering, Anna University, BIT Campus, Tiruchirappalli, India.
Nat Prod Res. 2021 Jul;35(13):2243-2248. doi: 10.1080/14786419.2019.1662012. Epub 2019 Sep 9.
A sensitive HPTLC method was developed for the simultaneous estimation of quercetin (QUR) and resveratrol (RES). The chromatographic separation was achieved using mobile phase toluene:chloroform:ethyl acetate:formic acid (3:2:4.9:0.1% v/v) and densitometric scan performed at 280 nm. The developed method was linear at 2-10 µg/mL with correlation coefficient of 0.9907 (QUR) and 0.9917 (RES). The method was validated for its precision, specificity, detection and quantification limits and % RSD was found to be less than 4.0%. The developed HPTLC method was evaluated in QUR and RES-loaded nanoformulation and leaf extract. The amount of QUR and RES present in the SG leaf extract was found to be 26.13 ± 0.7 µg/mg and 4.31 ± 0.8 µg/mg, respectively. The pH-dependent stability of RES has checked using the developed method. The above-developed method can be used to check the QUR/RES content in herbal/pharmaceutical formulation with scope towards industries.
建立了一种用于同时测定槲皮素（QUR）和白藜芦醇（RES）的灵敏 HPTLC 方法。采用甲苯：氯仿：乙酸乙酯：甲酸（3：2：4.9：0.1%v/v）作为流动相，在 280nm 处进行分光光度扫描，实现了色谱分离。该方法在 2-10μg/mL 范围内呈线性，相关系数分别为 0.9907（QUR）和 0.9917（RES）。该方法经过精密度、特异性、检测限和定量限验证，RSD 小于 4.0%。该 HPTLC 方法用于测定 QUR 和 RES 负载的纳米制剂和叶片提取物。SG 叶片提取物中 QUR 和 RES 的含量分别为 26.13±0.7μg/mg 和 4.31±0.8μg/mg。采用所建立的方法检查了 RES 的 pH 值依赖性稳定性。所建立的方法可用于检查草药/药物制剂中的 QUR/RES 含量，具有面向工业的应用前景。
