Wei Yameng, Liu Pengfei, Li Qian, Du Juan, Chen Yani, Wang Yu, Shi Haiyan, Wang Yanfeng, Zhang Huahua, Xue Wanjuan, Gao Yi, Li Dan, Feng Yun, Yan Jing, Han Jiming, Zhang Jing
Department of Clinical Medicine, Medical College of Yan'an University, Yan'an 716000, People's Republic of China.
Department of Gastroenterology, Yan'an University Affiliated Hospital, Yan'an 716000, People's Republic of China.
Onco Targets Ther. 2019 Aug 12;12:6361-6370. doi: 10.2147/OTT.S210800. eCollection 2019.
Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a tetramethylfolate dehydrogenase enzyme involved in folate metabolism. The aim of this study is to determine the effect of MTHFD2 on the proliferation and metastasis of colorectal cancer (CRC).
MTHFD2 was silenced or overexpressed in CRC cells. qRT-PCR and Western blotting were used to analyze mRNA and protein expression, respectively. The MTT assay and colony forming assay were used to detect cell proliferation and colony formation ability. The cycle and apoptosis changes were detected by flow cytometry. Transwell experiments were used to analyze the migration ability of CRC cells.
The expression of MTHFD2 in 31 kinds of cancers was analyzed by bioinformatics, and MTHFD2 was found highly expressed in various cancer cells including CRC cells. Silencing the expression of MTHFD2 resulted in inhibition of the proliferation of CRC cells, weakening of the migration ability, blocking of the cell cycle in G0/G1-S phase, and promotion of the apoptosis of CRC cells. On the contrary, overexpression of MTHFD2 in CRC cells resulted in enhancement of the proliferation and migration ability, promotion of cell cycle progression and inhibition of cell apoptosis.
MTHFD2 is positively related with colorectal cancer and the MTHFD2 gene is a tumor promoting gene in CRC cells.
亚甲基四氢叶酸脱氢酶2（MTHFD2）是一种参与叶酸代谢的四甲基叶酸脱氢酶。本研究旨在确定MTHFD2对结直肠癌（CRC）增殖和转移的影响。
在CRC细胞中沉默或过表达MTHFD2。分别采用qRT-PCR和蛋白质印迹法分析mRNA和蛋白质表达。采用MTT法和集落形成试验检测细胞增殖和集落形成能力。通过流式细胞术检测细胞周期和凋亡变化。采用Transwell实验分析CRC细胞的迁移能力。
通过生物信息学分析了31种癌症中MTHFD2的表达情况，发现MTHFD2在包括CRC细胞在内的各种癌细胞中高表达。沉默MTHFD2的表达导致CRC细胞增殖受到抑制，迁移能力减弱，细胞周期在G0/G1-S期受阻，并促进CRC细胞凋亡。相反，在CRC细胞中过表达MTHFD2导致增殖和迁移能力增强，促进细胞周期进程并抑制细胞凋亡。
MTHFD2与结直肠癌呈正相关，MTHFD2基因是CRC细胞中的促癌基因。
