基于流式细胞术检测壶腹周围癌或胰腺癌门静脉循环肿瘤细胞的术后转移预测
Postoperative metastasis prediction based on portal vein circulating tumor cells detected by flow cytometry in periampullary or pancreatic cancer.
作者信息
Tao Lianyuan, Su Li, Yuan Chunhui, Ma Zhaolai, Zhang Lingfu, Bo Shiping, Niu Yunyun, Lu Sijia, Xiu Dianrong
机构信息
Department of General Surgery, Peking University Third Hospital, Beijing 100191, People's Republic of China.
Department of Hepatobiliary Surgery, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, School of Clinical Medicine, Henan University, Zhengzhou, Henan 450003, People's Republic of China.
出版信息
Cancer Manag Res. 2019 Aug 6;11:7405-7425. doi: 10.2147/CMAR.S210332. eCollection 2019.
PURPOSE
The aim of this study was to evaluate the value of flow cytometry (FCM) detection of portal vein circulating tumor cells (CTCs) in predicting postoperative metastasis.
METHODS
Samples of portal venous blood and peripheral blood were collected from 39 patients during surgery, and CTCs were detected by FCM, with confirmation by laser confocal microscopy and single-cell sequencing.
RESULTS
Among all patients, a portal EpCAM+CD45- percentage ≥24.5×10 (=0.06), peripheral EpCAM+CD45- count ≥97/5 mL (=0.034), peripheral EpCAM+CD45- percentage ≥4.4×10 (=0.042), and CA242≥3.5 U/mL (=0.027) were significant predictors of metastasis. Further analysis showed that the portal EpCAM+CD45- ratio ≥24.5×10 is a predictor of metastasis (=0.025) in pancreatic cancer after curative resection.
CONCLUSION
CTCs detected by FCM in portal venous blood are of significant value for the prediction of postoperative metastasis in pancreatic or periampullary tumors.
目的
本研究旨在评估流式细胞术（FCM）检测门静脉循环肿瘤细胞（CTC）对预测术后转移的价值。
方法
在手术期间从39例患者采集门静脉血和外周血样本，通过FCM检测CTC，并经激光共聚焦显微镜和单细胞测序确认。
结果
在所有患者中，门静脉EpCAM+CD45-百分比≥24.5×10（=0.06）、外周血EpCAM+CD45-计数≥97/5 mL（=0.034）、外周血EpCAM+CD45-百分比≥4.4×10（=0.042）以及CA242≥3.5 U/mL（=0.027）是转移的显著预测指标。进一步分析表明，门静脉EpCAM+CD45-比率≥24.5×10是胰腺癌根治性切除术后转移的预测指标（=0.025）。
结论
FCM检测门静脉血中的CTC对预测胰腺或壶腹周围肿瘤术后转移具有重要价值。
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